Effect of Adding Aqueous Extract of Kalgan Seeds to Tris Buffer on Some Semen Characteristics of Holstein Bulls After Freezing
DOI:
https://doi.org/10.52783/jns.v14.1894Abstract
The study was conducted in the artificial insemination department from September 2024 to December 2024 with the aim of demonstrating effect of adding different concentrations of the aqueous extract of the seeds of the Kalgan plant to the Tris diluent on some traits of the semen of Holstein bulls after freezing. Four Holstein bulls were used in this experiment. Semen was collected from the four bulls every week at a rate of one ejaculation per bull. The semen of four bulls was mixed and mixed together (Pooled Semen) to eliminate individual differences between bulls. The experiment was divided into four treatments: The first treatment was the control treatment (T1) Tris diluted without adding antioxidant, T2: (Tris diluted + 501 μg of Kalgan seed extract / 10 ml diluted), T3: (Tris diluted + 250 μg of Kalgan seed extract / 10 ml diluted), (T4: Tris diluted + 350 μg of Kalgan seed extract / 10 ml diluted). The results showed that adding 250 μg of Kalgan seed aqueous extract / 10 ml / Tris diluted in treatment (T3) led to a highly significant increase (P>0.01) in the percentage of plasma membrane integrity compared to the rest of the treatments after freezing and liquefaction after 48 hours and after one month. The results also showed a highly significant decrease (P>0.01) in the concentration of malondialdehyde in the T3 treatment after freezing and liquefaction compared to the rest of the treatments. The results also showed a highly significant increase in the percentage of intact genetic material and the level of total antioxidants in the seminal plasma in T3 after freezing and liquefaction compared to the rest of the treatments. We conclude from this study that the addition of the aqueous extract of Kalgan seeds (250 μg/ml Tris diluted) led to an improvement in some semen traits after freezing of Holstein bulls (plasma membrane integrity, percentage of intact genetic material and level of total antioxidants in seminal plasma) and a decrease in the level of malondialdehyde in the seminal plasma.
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