Acute And Sub-Acute Toxicity Studies of a Poly Herbal Formulation Used for Management of Diabetes
DOI:
https://doi.org/10.63682/jns.v14i9S.2967Keywords:
Acute toxicity, Chronic toxicity, Poly-herbal formulation, Wistar ratsAbstract
The polyherbal solution comprising of Karela (Momordica charantia), Daruharidra (Berberis aristata), and Gudmar (Gymnema sylvestre), Amlatas (Cassis Fistula), Jamun (Syzyguium Cumin), Guduchi (Tinospora Cordifolia), Vijatsara (Pterocarpus Marsupium), Dalchini (Cinamomum Zeylenicum), Methi (Trigonella foenum graecum), Pipal (Piper Longum), Gum Acacia (Acacia Arabica) has been used for the management of Diabetes mellitus empirically. However, there are no available toxicity data for this combination of herbal formulation. Therefore, our study focused on evaluating the acute and subacute toxicity of this polyherbal formulation on Wistar rat model. Briefly the experiment was performed in two phases. The acute toxicity analysis was performed for 7days in 3 Wistar rats and sub-acute toxicity was performed on 9 Wistar rats for 28 days. For acute toxicity a single dose of 800 mg/kg and 1200mg/kg of polyherbal solution was administered to rats. While for sub-acute toxicity 2 doses variations of 100 mg and 200 mg administered orally for 28 days. Biochemical and histo-pathological analysis suggested negligible toxicity and no mortality was reported in both acute and sub-acute doses signifying non-toxicity of the poly-herbal formulation.
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Classification and Diagnosis of Diabetes: Standards of Medical Care in Diabetes—2020,” Diabetes Care, vol. 43, no. Supplement_1, pp. S14–S31, Jan. 2020, doi: 10.2337/dc20-S002.
“Introduction: Standards of Medical Care in Diabetes—2020,” Diabetes Care, vol. 43, no. Supplement_1, pp. S1–S2, Jan. 2020, doi: 10.2337/dc20-Sint.
L. Zeng, G. Zhang, Y. Liao, and D. Gong, “Inhibitory mechanism of morin on α-glucosidase and its anti-glycation properties,” Food Funct., vol. 7, no. 9, pp. 3953–3963, 2016, doi: 10.1039/C6FO00680A.
S. Parasuraman, G. Thing, and S. Dhanaraj, “Polyherbal formulation: Concept of ayurveda,” Pharmacogn. Rev., vol. 8, no. 16, p. 73, 2014, doi: 10.4103/0973-7847.134229.
P. Tiwari, B. N. Mishra, and N. S. Sangwan, “Phytochemical and Pharmacological Properties of Gymnema sylvestre : An Important Medicinal Plant,” Biomed Res. Int., vol. 2014, pp. 1–18, 2014, doi: 10.1155/2014/830285.
T. Tahir et al., “Therapeutic Uses and Pharmacological Properties of the Traditional South Asian Medicinal Plant Withania coagulans (Stocks) Dunal,” J. Herb. Med., vol. 47, p. 100926, Sep. 2024, doi: 10.1016/j.hermed.2024.100926.
S. Sharma et al., “The medicinal plant Berberis aristata and its endophytes for pharmacological applications: Current research and future challenges,” J. Appl. Biol. Biotechnol., 2024, doi: 10.7324/JABB.2024.167591.
A. Sharma, A. Kumar, and V. Jaitak, “Pharmacological and chemical potential of Cassia fistula L- a critical review,” J. Herb. Med., vol. 26, p. 100407, Apr. 2021, doi: 10.1016/j.hermed.2020.100407.
Payaam Vohra, Kundan Redekar, Sakshi Pawar, and Vaishali Patil, “Jamun: An Alchemy Botanical,” J. Ayurveda Integr. Med. Sci., vol. 9, no. 10, pp. 227–238, Jan. 2025, doi: 10.21760/jaims.9.10.38.
M. K. Rizvi et al., “Astounding Health Benefits of Jamun (Syzygium cumini) toward Metabolic Syndrome,” Molecules, vol. 27, no. 21, p. 7184, Oct. 2022, doi: 10.3390/molecules27217184.
R. Muckelbauer, G. Sarganas, A. Grüneis, and J. Müller-Nordhorn, “Association between water consumption and body weight outcomes: a systematic review,” Am. J. Clin. Nutr., vol. 98, no. 2, pp. 282–299, Aug. 2013, doi: 10.3945/ajcn.112.055061.
P. Bajaj, S. K. Chowdhury, R. Yucha, E. J. Kelly, and G. Xiao, “Emerging Kidney Models to Investigate Metabolism, Transport, and Toxicity of Drugs and Xenobiotics,” Drug Metab. Dispos., vol. 46, no. 11, pp. 1692–1702, Nov. 2018, doi: 10.1124/dmd.118.082958.
D. Singh et al., “A Comprehensive Review of Pathological Examination in Forensic Medicine: Past, Present, and Future.,” Cureus, vol. 14, no. 3, p. e22740, Mar. 2022, doi: 10.7759/cureus.22740
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