Phytochemical Analysis and In Vitro Anticancer Activity of Chloroform Extract of Centella Asiatica Leaves Using Lung Cancer (A549)
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Medicinal plants are a cornerstone of traditional medicine and are extensively studied for their therapeutic properties. Among them, Centella asiatica is well known for its diverse pharmacological benefits, including its potential anticancer properties. This study aims to evaluate the phytochemical composition and in vitro anticancer activity of chloroform extract derived from the leaves of C. asiatica against the lung cancer cell line A549. The phytochemical screening revealed the presence of key bioactive compounds such as alkaloids, flavonoids, glycosides, carbohydrates, and amino acids, which are known for their biological and therapeutic effects.
The anticancer potential of the chloroform extract was assessed using the MTT assay, which measures cellular viability by evaluating mitochondrial activity. The extract demonstrated significant dose-dependent cytotoxicity against the A549 cell line, with IC50 values indicating its potency. The results highlight the potential of C. asiatica to inhibit cancer cell proliferation and induce cytotoxicity, making it a promising candidate for further development as a natural anticancer agent.
Phytochemicals such as flavonoids and alkaloids, which were detected in the extract, have been extensively reported in the literature for their roles in inducing apoptosis, preventing metastasis, and exhibiting antioxidant activity. These properties likely contribute to the extract's anticancer effects observed in this study. Additionally, the presence of glycosides and amino acids may support complementary mechanisms in targeting cancer cells.
The findings of this study underscore the potential of C. asiatica as a natural source for anticancer agents, particularly in the context of lung cancer. Future studies should focus on isolating and characterizing specific active compounds, determining their precise mechanisms of action, and conducting in vivo experiments to validate their efficacy. This research also emphasizes the importance of medicinal plants as a sustainable and accessible resource for drug discovery, offering an alternative to synthetic drugs, which often have significant side effects
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Ahn, K. (2017). The worldwide trend of using botanical drugs and strategies for developing global drugs. BMB Reports, 50(3), 111–116.
Sharma, A. (2017). Medicinal plants and their role in primary healthcare. Journal of Ethnopharmacology, 204, 42–55.
Singh, P., et al. (2017). Phytochemicals in traditional and modern medicine. Advances in Pharmacology, 72, 23–45.
Kumar, S., et al. (2013). Natural compounds as epigenetic regulators in cancer therapy. Oncology Letters, 6(3), 924–931.
Jemal, A., et al. (2011). Global cancer statistics. CA: A Cancer Journal for Clinicians, 61(2), 69–90.
Moghadamtous, S. Z., et al. (2015). Medicinal plants for the treatment of cancer: A comprehensive review. Journal of Cancer Research and Therapy, 11(4), 483–496.
Bauer, A., & Brönstrup, M. (2014). Industrial natural product chemistry for drug discovery and development. Natural Product Reports, 31(1), 35–60.
Shakya, A. K. (2016). Medicinal plants: Future source of new drugs. International Journal of Herbal Medicine, 4(4), 59–64.
Kennedy, D. O. (2011). Phytochemicals: Roles in health and disease. Nutritional Bulletin, 36(1), 64–72.
Prakash, R. (2015). Himalayan medicinal plants and their role in traditional healthcare. Journal of Ethnopharmacology, 165, 123–142.
World Health Organization. (2013). Traditional medicine: Factsheet. Geneva: WHO Press.
Cragg, G. M., & Newman, D. J. (2013). Natural products: A continuing source of novel drug leads. Biochimica et Biophysica Acta (BBA), 1830(6), 3670–3695.
Ngoci Njeru, S. (2013). Phytochemicals: Role in health and disease. Journal of Medicinal Plant Research, 7(13), 834–846.
Chen, L. (2007). Dietary phytochemicals and chronic disease prevention. Annual Review of Nutrition, 27, 81–103.
Temple, N. J., et al. (2003). Fruit and vegetable consumption and cancer prevention. Nutrition Research Reviews, 16(2), 167–182.
Price, K. R., et al. (1992). Role of phytochemicals in food science. Journal of the Science of Food and Agriculture, 58(3), 377–384.
Kharchouf, S., et al. (2017). Advances in phytochemical analysis of medicinal plants. Phytochemistry Reviews, 16(4), 615–628.
Caroling, G., et al. (2015). Emerging methods in phytochemical studies. Journal of Pharmacognosy and Phytochemistry, 4(2), 87–94.
Aggarwal, B. B., et al. (2009). Anti-inflammatory properties of dietary phytochemicals. Nutrition and Cancer, 61(6), 800–810.
Chen, Z., et al. (2019). Isoflavones and bone health: Molecular insights. Current Osteoporosis Reports, 17(3), 175–182.
Sagbo, I. J., et al. (2017). Phytochemicals as antibacterial agents. Biomedicine and Pharmacotherapy, 96, 150–158.
Elumalai, K., et al. (2016). Role of phytochemicals in human health. Advances in Pharmacology and Toxicology, 17(1), 57–66.
Matsuura, H., et al. (2015). Alkaloid accumulation in plants and their ecological roles. Plant Cell Reports, 34(8), 1261–1271.
Panche, A. N., et al. (2016). Flavonoids: A comprehensive review of therapeutic properties. Journal of Advanced Research, 7(2), 123–132.
Desai, S., et al. (2009). Saponins and their biological effects. Current Pharmaceutical Design, 15(11), 1203–1215.
Ferrer-Gago, F. J., et al. (2019). Historical and modern uses of resins in natural products chemistry. Natural Product Reports, 36(8), 985–1003.
Moore, D. R. (2015). Protein metabolism and requirements in health and disease. Annual Review of Nutrition, 35, 539–565.
Ochwang’I, D. O., et al. (2014). Medicinal plants and their potential in anticancer treatment. Journal of Medicinal Plants.
Sivaraj, R., et al. (2014). Role of plant extracts in nanomaterial drug development. Cancer Research Journal.
Schnekenburger, M., et al. (2014). Epigenetic drugs and their potential in cancer therapy. Epigenetics & Cancer Journal.
Rajeswara Rao, B. R., et al. (2007). Phytochemicals with anticancer potential. World Health Organization (WHO) report.
Amin, A., et al. (2009). Plant-derived anticancer drugs and their mechanisms of action. Cancer Therapy Reviews.
Khazir, J., et al. (2014). Taxane derivatives in cancer treatment. Chemotherapy Advances.
Parveen, S., et al. (2013). Conservation of medicinal plants: Importance and methods. Medicinal Plant Conservation Journal.
Zschocke, S., et al. (2000). Threats to medicinal plant species and methods for their conservation. Environmental Biology Journal.
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