Touch DNA technique and its utility in Criminal Justice System
Keywords:
Touch DNA, DNA profiling, forensic science, Polymerase Chain reactionAbstract
DNA analysis or DNA profiling done on the samples available from the crime scenes is considered the most important evidence in present criminal justice system. The type of samples on which this analysis are done are blood, semen, body fluids etc. But lack of this sample and lack of proper quantity can be a problem in solving cases which then are labelled as cold cases. There has been many up gradation and research in DNA profiling and of them the most important is Touch DNA analysis. The advantages of this technique like possibilities of analysis of small quantities of sample, collection of DNA from areas of touch like surfaces, clothes, glasswares, mobiles, personal computers etc. has led to solving cold case and complicated cases. Various techniques of touch DNA have their share of challenges. There is constant research and development in new modalities in touch DNA. In present times throughout the globe touch DNA has generated huge interest. In this review we present the overview of the Touch DNA technique and its utility in criminology navigating almost all the aspects including the latest developments, accuracy, advantages, challenges, implications of global use and future perspectives.
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Haddrill PR. Developments in forensic DNA analysis. Emerg Top Life Sci [Internet]. 2021;5(3):381–93. Available from: http://dx.doi.org/10.1042/etls20200304
Tozzo P, Mazzobel E, Marcante B, Delicati A, Caenazzo L. Touch DNA sampling methods: Efficacy evaluation and systematic review. Int J Mol Sci [Internet]. 2022;23(24):15541. Available from: http://dx.doi.org/10.3390/ijms232415541
van Oorschot RAH, Ballantyne KN, Mitchell RJ. Forensic trace DNA: a review. Investig Genet [Internet]. 2010;1(1):14. Available from: http://dx.doi.org/10.1186/2041-2223-1-14
Sessa F, Salerno M, Bertozzi G, Messina G, Ricci P, Ledda C, et al. Touch DNA: impact of handling time on touch deposit and evaluation of different recovery techniques: An experimental study. Sci Rep [Internet]. 2019;9(1). Available from: http://dx.doi.org/10.1038/s41598-019-46051-9
Bhandari D. Touch DNA: Revolutionizing evidentiary DNA forensics. International Journal of Forensic Sciences [Internet]. 2023;8(3):1–8. Available from: http://dx.doi.org/10.23880/ijfsc-16000314
Rutty GN, Hopwood A, Tucker V. The effectiveness of protective clothing in the reduction of potential DNA contamination of the scene of crime. Int J Legal Med [Internet]. 2003;117(3):170–4. Available from: http://dx.doi.org/10.1007/s00414-002-0348-1
Basset P, Castella V. Lessons from a study of DNA contaminations from police services and forensic laboratories in Switzerland. Forensic Sci Int Genet [Internet]. 2018;33:147–54. Available from: http://dx.doi.org/10.1016/j.fsigen.2017.12.012
Meakin G, Jamieson A. DNA transfer: Review and implications for casework. Forensic Sci Int Genet [Internet]. 2013;7(4):434–43. Available from: http://dx.doi.org/10.1016/j.fsigen.2013.03.013
Alketbi SK. An Innovative Solution to Collect Touch DNA for Direct Amplification. 2022.
Butler JM. Recent advances in forensic biology and forensic DNA typing: INTERPOL review 2019–2022. Forensic Sci Int Synerg [Internet]. 2023;6(100311):100311. Available from: http://dx.doi.org/10.1016/j.fsisyn.2022.100311
Liu JY. Direct qPCR quantification of unprocessed forensic casework samples. Forensic Sci Int Genet [Internet]. 2014;11:96–104. Available from: http://dx.doi.org/10.1016/j.fsigen.2014.03.003
Gammon K, Murray-Jones K, Shenton D, Wood Z, Mayers C. Touch DNA on objects can be analysed at low cost using simplified direct amplification methods [Internet]. bioRxiv. 2019. Available from: http://dx.doi.org/10.1101/540823
Meakin GE, Butcher EV, van Oorschot RAH, Morgan RM. Trace DNA evidence dynamics: An investigation into the deposition and persistence of directly- and indirectly-transferred DNA on regularly-used knives. Forensic Sci Int Genet [Internet]. 2017;29:38–47. Available from: http://dx.doi.org/10.1016/j.fsigen.2017.03.016
Jansson L, Swensson M, Gifvars E, Hedell R, Forsberg C, Ansell R, et al. Individual shedder status and the origin of touch DNA. Forensic Sci Int Genet [Internet]. 2022;56(102626):102626. Available from: http://dx.doi.org/10.1016/j.fsigen.2021.102626
Kanokwongnuwut P, Kirkbride KP, Kobus H, Linacre A. Enhancement of fingermarks and visualizing DNA. Forensic Sci Int [Internet]. 2019;300:99–105. Available from: http://dx.doi.org/10.1016/j.forsciint.2019.04.035
Zhang J, Liu W, Simayijiang H, Hu P, Yan J. Application of microbiome in forensics. Genomics Proteomics Bioinformatics [Internet]. 2023;21(1):97–107. Available from: http://dx.doi.org/10.1016/j.gpb.2022.07.007
Alvarez-Cubero MJ, Saiz M, Martínez-García B, Sayalero SM, Entrala C, Lorente JA, et al. Next generation sequencing: an application in forensic sciences? Ann Hum Biol [Internet]. 2017;44(7):581–92. Available from: http://dx.doi.org/10.1080/03014460.2017.1375155
Yang Y, Xie B, Yan J. Application of next-generation sequencing technology in forensic science. Genomics Proteomics Bioinformatics [Internet]. 2014;12(5):190–7. Available from: http://dx.doi.org/10.1016/j.gpb.2014.09.001
Glynn CL. Bridging disciplines to form a new one: The emergence of forensic Genetic Genealogy. Genes (Basel) [Internet]. 2022;13(8):1381. Available from: http://dx.doi.org/10.3390/genes13081381
Yudianto A, Nuraini M I, Furqoni AH, Nzilibili SMM, Harjanto P. The use of touch DNA analysis in forensic identification focusing on Short Tandem Repeat-Combined DNA Index System loci THO1, CSF1PO and TPOX. Infect Dis Rep [Internet]. 2020;12(11):8716. Available from: http://dx.doi.org/10.4081/idr.2020.8716
Schulte J, Rittiner N, Seiberle I, Kron S, Schulz I. Collecting touch DNA from glass surfaces using different sampling solutions and volumes: Immediate and storage effects on genetic STR analysis. J Forensic Sci [Internet]. 2023;68(4):1133–47. Available from: http://dx.doi.org/10.1111/1556-4029.15305
Yudianto A, Simon, Simon, Setiawan F. The Use of Touch DNA Analysis in Forensic Identification Focusing on STR CODIS LOCI THO1. 2022.
Bruijns B, van Asten A, Tiggelaar R, Gardeniers H. Microfluidic devices for forensic DNA analysis: A review. Biosensors (Basel) [Internet]. 2016;6(3):41. Available from: http://dx.doi.org/10.3390/bios6030041
Horsman KM, Bienvenue JM, Blasier KR, Landers JP. Forensic DNA analysis on microfluidic devices: A review. J Forensic Sci [Internet]. 2007;52(4):784–99. Available from: http://dx.doi.org/10.1111/j.1556-4029.2007.00468.x
Walsh S, Liu F, Wollstein A, Kovatsi L, Ralf A, Kosiniak-Kamysz A, et al. The HIrisPlex system for simultaneous prediction of hair and eye colour from DNA. Forensic Sci Int Genet [Internet]. 2013;7(1):98–115. Available from: http://dx.doi.org/10.1016/j.fsigen.2012.07.005
Yang J, Lin D, Deng C, Li Z, Pu Y, Yu Y, et al. The advances in DNA mixture interpretation. Forensic Sci Int [Internet]. 2019;301:101–6. Available from: http://dx.doi.org/10.1016/j.forsciint.2019.05.024
Haned H, Benschop CCG, Gill PD, Sijen T. Complex DNA mixture analysis in a forensic context: Evaluating the probative value using a likelihood ratio model. Forensic Sci Int Genet [Internet]. 2015;16:17–25. Available from: http://dx.doi.org/10.1016/j.fsigen.2014.11.014
National Research Council (US) Committee on DNA Forensic Science: An Update. Ensuring high standards of laboratory performance. Washington, D.C., DC: National Academies Press; 1996.
Morgan AG, Prinz M. Development of improved DNA collection and extraction methods for handled documents. Genes (Basel) [Internet]. 2023;14(3):761. Available from: http://dx.doi.org/10.3390/genes14030761
Valentine JL, Presler-Jur P, Mills H, Miles S. Evidence collection and analysis for touch deoxyribonucleic acid in groping and sexual assault cases. J Forensic Nurs [Internet]. 2021;17(2):67–75. Available from: http://dx.doi.org/10.1097/jfn.0000000000000324
Fonneløp AE, Johannessen H, Egeland T, Gill P. Contamination during criminal investigation: Detecting police contamination and secondary DNA transfer from evidence bags. Forensic Sci Int Genet [Internet]. 2016;23:121–9. Available from: http://dx.doi.org/10.1016/j.fsigen.2016.04.003
Abdel Hady RH, Thabet HZ, Ebrahem NE, Yassa HA. Thermal effects on DNA degradation in blood and seminal stains: Forensic view. Acad Forensic Pathol [Internet]. 2021;11(1):7–23. Available from: http://dx.doi.org/10.1177/1925362121998547
Alketbi SK, Goodwin W. The effect of time and environmental conditions on Touch DNA. Forensic Sci Int Genet Suppl Ser [Internet]. 2019;7(1):701–3. Available from: http://dx.doi.org/10.1016/j.fsigss.2019.10.144
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