Barr Bodies Revelation from Dental Pulp – A Comparative Study on the Basis Of X- Chromatin Visibility and Nuclear Membrane Clarity
Keywords:
Dental Pulp, Inactivated X-chromosome, Sex Chromatin, tissue processing, cytological, nuclear membrane, X-chromatin, genderAbstract
Background: A convex or triangular shaped dynamic inactivated X chromosomal configuration is seen in female somatic cell nuclei. These morphologic variants are termed as Sex chromatin or barr bodies. Determination of gender is of valuable significance especially in cases where bodies are charred/ burnt and are beyond positive identification. Discovered by Murray Barr, these bodies are inactivated by a process known as Lyonization. Dental pulp, blood, hair follicles buccal smears etc proves to be impending sources from which barr bodies can be obtained.
Aims and objectives: The main aim and objective of the study is to assess barr bodies on the basis of X- chromatin visibility and nuclear membrane. Intraobserver variability of barr bodies were also evaluated and both histopathological and cytopathological methods were also evaluated
Material and methods: Total number of 60 subjects i.e. extracted teeth within the age group 10-40 years from 20 male & 40 females were taken. Dental pulp was obtained from the extracted teeth. Total number of two respective groups, Group 1 and Group II were categorized which included 10 males & 20 females each. Rapid tissue processing was done in group I and in Group II cytological method using centrifugation was inculcated.
Results: Nuclear membrane was extremely noticeable in rapid tissue processing histopathological method samples both in males and females. Conversely, in cytological method, the clarity of membrane was very indistinct. In Histopathological females of group 1, X-chromatin visibility was of paramount excellence. In case of males of both group I and II, X- chromatin was poorly visualized. A statistically significant difference was obtained
Conclusion: Nuclear membrane and X- chromatin visibility were superior in histopathological method especially in case of females. Histopathological method was found to be more feasible in revelation of barr bodies for medico- legal purposes
Downloads
Metrics
References
SweetD. Why a dentist for identification? DentClin North Am 2001;45:237-51
Mittwoch U. Sex Chromatin. JMedGened 1964;1(1):69-76
Mittal T, Saralaya KM, Kuruvilla A, Achary C. Sex determination from buccal mucosa scrapes. Int J Legal Med 2009; 123: 437 40.
Mornstad H, Pleiffer H, Yoon C, TeivansA. Demonstration & semi-quantification of mtDNA from human dentin & its relation to age.Int J legal Med 1999; 112:98-100
Zagga AD, Ismail SM, Ahmed H, Tadros AA. Forensic study for genetic sex determination of burnt powdered skeletal fragments from sokoto, northwestern Nigeria. IOSR J Dent med sci 2013; 7:47-54.
Garm S.M Sex difference in tooth size. J Dent. Res., 1964; 43:306
GondaK. The sex difference in the size of teeth.J.Anthropol 1959; 67:151-63 Ata –Ali J, Ata –Ali F. Forensic dentistry in human identification: A review of the literature. JclinExp Dent. 2014; 162-67.
Sharma S, Singh V,Chatterjee S, Singh H & Singh D. Comparative Analysis of Techniques for Sex Determination through Dental Pulp Barr Bodies: Forensic Study. Int J curr res 2016; 8(8):37204-8.
Bhardwaj N, Nangia R, Puri A, Bhat N, Wadhwan V, Gupta H. Determination of gender from dental pulp by identification of Barr bodies: A comparative study. J Oral Maxillofac Pathol 2022; 26:488-94.
Barr M L & Bertram E G. Nature London 1949; 163:676-77.
Bhardwaj N, Bhat N et al. Barr Bodies –A Spoonful of Sex Chromatin. International Journal of current medical and pharmaceutical research 2020; 6(01):4939-42
Baby TK, Thomas P, Palani J, Pillai RK, Ramakrishnan BP. Sex determination efficacy of Papanicolaou and acriflavine Schiff stains in buccal smears. J Forensic Dent Sci 2017; 9:46-7.
Johnson J, Raheese J. A Comparative Study of Aceto - Orcein and Papancolaeu Staining Methods for the Demonstration and Enumeration of Barr body in Buccal Smears. International Journal for Research in Applied Science & Engineering Technology (IJRASET).2018 July; 6(7) 2321-9653
...
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
