Total Flavonoids, Total Phenolics and Targeted HPTLC Profile for Quantification of Quercetin in Wedelia chinensis and Cassytha filiformis
DOI:
https://doi.org/10.63682/jns.v14i24S.5964Keywords:
Hydroalcoholic, Phenolics, Flavonoids, Quercetin, HPTLCAbstract
This study quantitatively determined the total phenolic and flavonoid contents in hydroalcoholic extracts of Wedelia chinensis and Cassytha filiformis using a spectrophotometric method. We also performed an HPTLC profile for both plant extracts to determine the quantity of quercetin. A sensitive and reliable high-performance thin-layer chromatographic method has been developed to quantify quercetin in dried whole plant powder. Wedelia chinensis and Cassytha filiformis hydroalcoholic extract was chromatographed on silica gel 60 F254 plates. The linear ascending development was carried out in a twin trough glass chamber (CAMAG, Muttenz, Switzerland) with Toluene: Acetone: Formic Acid (4.5:4.5:1) (v/v/v), as mobile phase. Total phenolic content was found to be (155 mg of GAE/g of crude extract) in the Wedelia chinensis, and the lowest was in the Cassytha filiformis extract (52.01 mg of GAE/g). Wedelia chinensis also had the highest total flavonoid content (72.3 ± 0.233 mgQE/g), while the lowest was in the Cassytha filiformis (3.19± 0.256 mgQE/g). In this research, the Rf values of quercetin were found to be 0.75 and 0.76 in Wedelia chinensis and Cassytha filiformis, respectively.
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