Biocontrol Potential of Actinomycetes Against Xanthomonas-Induced Oily Spot in Pomegranate
Keywords:
antagonistic actinomycetes, Xanthomonas axonopodis pv., punicae, oily spot disease, biocontrol, pomegranate,, etcAbstract
Punica granatum (Pomegranate) faces significant challenges due to diseases such as oily spot caused by the bacterium Xanthomonas axonopodis pv. punicae, which severely affects fruits quality and yield. Actinomycetes are known for their versatility in production of antagonistic compounds. This study aimed at isolation and characterization of antagonistic actinomycetes from natural sources for controlling oily spot disease in pomegranates. Soil samples were collected from diseased orchards and other natural habitats like the sites where spoiled and waste pomegranates and peels are dumped. Serial dilution technique and Glycerol Aspargine agar and Actinomycete agar were used for isolation and screening of the actinomycetes against the pathogen. The pathogen of oil spots was isolated from alcohol disinfected spots and using Potato dextrose agar, Starch agar. Promising strains of actinomycetes demonstrated significant inhibition of the pathogen. The isolates were characterized based on their colony morphology, pigmentation, spore pattern and biochemical properties. The findings suggest that certain actinomycetes (Streptomyces spp. and Micromonospora spp.) have potential as biocontrol agents offering an eco- friendly alternative to chemical treatments for managing oily spot disease in pomegranates. Further study is in progress to optimize the use of these strains in sustainable agricultural practices.
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