In Vitro Microleakage of Fissure Sealant Following Enamel Surface Treatment with Sodium Hypochlorite and Application of a Universal Adhesive
Keywords:
Pit and Fissure Sealants, Sodium Hypochlorite, Dental LeakageAbstract
Objectives: This study aimed to assess the microleakage of fissure sealant following enamel surface treatment with 5.25% sodium hypochlorite (NaOCl) and application of a universal adhesive.
Materials and Methods: In this in vitro study, 75 sound extracted premolars were randomly assigned to 5 groups (n=15) of (I) phosphoric acid etching + sealant (control), (II) NaOCl + etching + sealant, (III) etching + G-Premio Bond application, (IV) NaOCl + etching + G-Premio Bond, and (V) G-Premio Bond + sealant. All groups were then incubated for 24 hours, and underwent 3000 thermal cycles. They were subsequently immersed in 50% silver nitrate and sectioned buccolingually. The dye penetration depth was measured under a light microscope to determine the microleakage score. Data were analyzed by the Kruskal-Wallis and Dunn tests (alpha=0.05).
Results: The microleakage was significantly different among the five groups (P<0.05). Application of universal adhesive (groups III, IV and V) significantly increased the microleakage. Application of NaOCl for enamel preparation decreased the microleakage compared with the groups without NaOCl application. The lowest microleakage was recorded in group II (NaOCl + etching + sealant).
Conclusion: Although the difference in microleakage was not significant between the NaOCl and the control groups, lower microleakage in NaOCl groups suggests that enamel surface treatment with NaOCl may decrease microleakage under certain circumstances. Application of G-Premio Bond under sealant increased the microleakage, and is therefore not recommended for microleakage reduction.
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