Formulation and Evaluation of Flavanosomes Encapsulated with Sesamol and Curcumin

Abstract

The objective of the present study was to prepare flavonosomes loaded with sesamol and curcumin in order to improve the bioavailability of the flavonoids. The flavonosomes loaded with sesamol and curcumin were prepared using bulk co-loading method using lecithin as the lipid molecule. The particle size of the flavonosomes was determined using zeta sizer and ranged from 501.42 nm to 167.08 nm. The entrapment efficiency of F4 has been calculated to be 93.12%. The flavonosome F4 was studied for release of the entrapped flavonoids by dialysis method. After the first hour, the entrapped flavonoids released in a steady manner from the flavonosome depicting and almost complete release (88.27% for sesamol and 93.66% for curcumin) from the formulation. The best formulation with respect to particle size and anti-oxidant action was F4 that contained 4:1 ratio of lecithin: flavonoids. DPPH radical scavenging assay was used to determine the antioxidant action of the individual flavonoids and the flavonosome formulation. It was found that the formulation F4 was having higher antioxidant activity in comparison to sesamol and curcumin. The IC₅₀ of sesamol, curcumin and flavonosome were found to be 233.76 µg/mL, 181.87 µg/mL and 164.30 µg/mL respectively. The results led to conclude that the prepared flavonosomes present better stability, prolonged release and good anti-oxidant activity