Correlation of Red Blood Cells Storage Lesion F2α-Isoprostanes Levels with Erythrocyte-Derived Microparticles
Keywords:
red blood cells storage lesions, erythrocyte-derived microparticles (EMP), F2α-isoprostanesAbstract
Background: PRC stored blood products change morphology, biochemistry, and metabolism, called storage lesion red blood cells (erythrocyte storage lesion). Morphological changes of erythrocyte membrane phospholipids and damage due to oxidative stress and hemolysis allegedly produce microparticles known as erythrocyte-derived microparticles (EMP) and F2α-isoprostanes. F2α-isoprostanes are the indicators used to assess lipid peroxidation and cellular oxidative stress. Increased EMP-isoprostanes F2α in the PRC product is allegedly one of the causes of bad outcomes in transfusions.
Aim: to know the correlation between red blood cells storage lesions (F2α-isoprostane) with erythrocyte-derived microparticles (CD235a) in the PRC product based storage time, days 0, 7,14,21, and 28 at the blood banks Hospital Dr. M. Djamil Padang. Methods: The cross-sectional study of 14 PRC units came from 14 healthy donors at the Blood Bank of Dr. M.Djamil Padang Hospital. The study lasted from May 2016 to August 2017. The tests were conducted during 28 days of storage at 1-week intervals. Examination of the level F2α-isoprostane with the methods Enzyme-linked immunosorbent assay (ELISA) and examination of EMP levels (CD235a) with the flow cytometry method. Bivariate statistical analysis of the correlation parameter storage lesion red blood cells (F2α-isoprostanes) with EMP (CD235a) using the Pearson correlation test with a P value <0.05 indicates exhibited significant correlation. Results: Fourteen units of PRC obtained the largest donors were male (85.7%), with the highest blood type being O (42.9%). During storage, there is an increased amount of F2α-isoprostane and erythrocyte-derived microparticles (EMP) in the PRC product. There was no statistically significant correlation between the level of F2α-isoprostane F2α and the amount of EMP during storage up to 28 days.
Conclusion: There was no statistically significant correlation between the level of isoprostane F2α and the amount of EMP in the storage PRC
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