Metabolomic Profiling And In-Vitro Antipsoriatic Potential Of Jasminum Auriculatum Extracts
Keywords:
Antipsoriatic, HaCaT, Jasminum auriculatum, In-vitro cell lines, Metabolic profiling, IC50Abstract
Objective: To scientifically validate the antipsoriatic activity of chloroform and methanolic extracts derived from Jasminum auriculatum (whole plant), traditionally used in Ayurveda and ethnomedicine for treating skin ailments.
Methods: Chloroform and methanolic extracts of Jasminum auriculatum were prepared and subjected to metabolomic profiling and phytochemical characterization using LC-MS. Preliminary phytochemical screening was also conducted. The in-vitro antipsoriatic activity was evaluated using the HaCaT human keratinocyte cell line via the MTT assay. Additionally, lipoxygenase and nitric oxide (NO) inhibition assays were performed to assess anti-inflammatory potential.
Results: LC-MS and phytochemical screening revealed the presence of phenolic compounds, flavonoids, sterols, and essential fatty acids. Both extracts exhibited concentration-dependent inhibition of HaCaT cell proliferation, with IC₅₀ values of 88.02 µg/ml (chloroform extract) and 32.46 µg/ml (methanolic extract). The extracts also demonstrated notable lipoxygenase and NO inhibitory activity. Among the two, the chloroform extract showed superior antipsoriatic effects in the HaCaT cell line study.
Conclusion: Jasminum auriculatum extracts, especially the chloroform extract, contain potent bioactive compounds with significant antipsoriatic activity. These findings scientifically support the traditional use of the plant in managing psoriasis and highlight its potential in developing effective phytotherapeutic treatments.
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