Immunogenicity Response Test of Human Peripheral Blood Mononuclear Cells Culture After Application of Scaffold Freeze-Dried Bovine Bone, Decellularized Freeze-Dried Bovine Bone, and Deproteinized Bovine Bone Materials (In Vitro Laboratory Observational Re
DOI:
https://doi.org/10.63682/jns.v14i8.6818Keywords:
Scaffold, Cytokine, Interleukin, Secretion, ImmunityAbstract
Bovine bone xenograft's physical and chemical structure is similar to human bone; therefore, it has been a choice of bone graft material other than autograft. Freeze-dried bovine bone (FDBB) scaffolds with or without decellularization still have organic content and will react to the body's immunity and trigger an inflammatory response. At the same time, Deproteinized Bovine Bone Material (DBBM) has been widely used because it is not antigenic. Peripheral Blood Mononuclear Cell culture was used to prove the inflammatory response through cytokine secretion levels of Interleukin-1 and Interleukin-10. This research aims to evaluate and compare IL-1 and IL-10 secretion levels as inflammatory markers in FDBB, dc-FDBB and DBBM scaffolds. The method used in this research are FDBB, dc-FDBB, and DBBM scaffolds were observed in human Peripheral Blood Mononuclear Cells conditioned medium for 1, 3, and 7 days to see the comparison of IL-1 and IL-10 secretion levels by ELISA using an ELISA Reader at 450 nm absorbent with a colour change to intense yellow. In addition, observations of Optical Density results were made on the three scaffolds. Furthermore, a data distribution test was conducted with Shapiro-Wilk and data analysis with ANOVA. The results in the observation of Interleukin-1 cytokine secretion levels show that FDBB and DBBM scaffolds have decreased secretion levels from day 1, 3 to 7, but dc-FDBB scaffolds appear to have increased Interleukin 1 secretion levels on day three, but decreased on day 7. In the observation of IL-10 secretion, both FDBB, dc-FDBB, and DBBM scaffolds experienced a significant decrease in secretion levels compared to the control group. There was no significant difference between the FDBB, dc-FDBB and DBBM groups on days 1, 3 and 7 of observation (p>0.05). The observations showed that the three scaffolds were not antigenic.
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