UV Spectrophotometric Method Development And Validation For The Combined Plant Extract Traditionally Used In Arunachal Pradesh To Treat Malaria

Abstract

This study successfully developed and validated a simple, precise, and highly sensitive UV spectrophotometric method for the comprehensive analysis and quality control of combined plant extracts. Traditional medicine practitioners in Arunachal Pradesh, India, use a combination of Coptis teeta wall., Andrographis paniculata, and Nyctanthes arbor-tris to treat malaria. Hydroalcoholic extract of these three plants were prepared and combined in a 5:3:2 ratio. UV-visible double beam spectrophotometer method was used for determining maximum absorption wavelengths (λ_max) at 230nm for ethanol, 203nm for Phosphate buffer solution (PBs) (p^H 7.4), and 224nm for acidic buffer solution (ABs) (p^H 1.2). The developed method was rigorously validated according to ICH guidelines for linearity, precision, accuracy, LOD, and LOQ. The results have shown excellent linearity with high correlation coefficients (R²> 0.9980 across various concentration ranges (5-60 µg/ml for ethanol and PBs, 5-90 µg/ml for ABs). Precision was confirmed through low relative standard deviation values for both intra-day and inter-day measurements. Accuracy was demonstrated by high percentage recovery values, affirming minimal error. The method also exhibited high sensitivity, with low LOD and LOQ values, enabling the detection and quantification of trace amounts of the analytes. The validated UV spectrophotometric method provides a rapid, cost-effective, and dependable tool for ensuring the identity, batch-to-batchg quality control evaluations and pharmacokinetic studies.