Phytochemical Screening and Cytotoxic Potential of Lantana camara Leaf Extract against MCF-7 Cells: A Cell Morphology and MTT Assay Approach
DOI:
https://doi.org/10.63682/jns.v14i22S.7537Keywords:
Lantana camara, Acetone extract, MCF-7 cells, Cytotoxicity, MTT assay, Cell morphologyAbstract
Objective: The present study aimed to evaluate the cytotoxic potential of Lantana camara leaf extract against MCF-7 human breast cancer cells using the MTT assay and morphological analysis.
Methods: Dried leaves of Lantana camara were extracted using acetone in a Soxhlet apparatus.
Phytochemical Screening: The crude extract was screened for secondary metabolites, which confirmed the presence of flavonoids, alkaloids, phenolics, and terpenoids.
Cell Culture and Treatment: MCF-7 cells were cultured in a 96-well plate and treated with various concentrations (50, 100, and 250 μg/mL) of the extract after 12 hours of incubation.
Cytotoxicity Evaluation: Cell morphology was observed microscopically, and MTT assay was performed at 24, 48, and 72 hours post-treatment to assess cytotoxicity.
Results: The extract exhibited time- and dose-dependent cytotoxic effects. Significant morphological alterations were observed at
250 μg/mL. The IC₅₀ values were determined to be 153.54 ± 0.85 μg/mL (24 h), 147.16 ± 1.13 μg/mL (48 h), and 141.98 ± 1.15 μg/mL (72 h), suggesting progressive cytotoxicity with extended exposure.
Conclusion: The findings support the potential anticancer activity of Lantana camara, likely due to its rich phytochemical content. Further studies are recommended to isolate active compounds and validate their efficacy in vivo
Downloads
Metrics
References
A.Pal, S Sanyal, S. Das, T.K. Sengupta Effect of Lantana camara Ethanolic Leaf Extract on Survival and Migration of MDA-MB-231 Triple-Negative Breast Cancer Cell Line. Journal of Herbal Medicine Volume 43, February 2024, 100837.
Vishwanadham, Y., Madhuri, R., Shivaraj, Appaji, Kavita Waghray., Targeting PI3K/AKT/mTOR Signaling with Novel Andrographolide Analogues Potentially Induces Apoptosis and Inhibits the Metastatic Ability of Human Breast Cancer Cells: Rational Design, Synthesis, and In Vitro Studies. Russ J Bioorg Chem 51, 601–628 (2025). https://doi.org/10.1134/S106816202460363X.
N.J.P. Subhashini, E. Praveen Kumar, Nirmala Gurrapu, Vishwanadham Yerragunta, Design and synthesis of imidazolo-1, 2,3-triazoles hybrid compounds by microwave-assisted method: Evaluation as an antioxidant and antimicrobial agents and molecular docking studies,Journal of Molecular Structure, Volume 1180, 2019, Pages 618-628, ISSN 0022-2860, https://doi.org/10.1016/j.molstruc.2018.11.029.
E. Praveenkumar, Nirmala Gurrapu, Prashanth Kumar Kolluri, Vishwanadham Yerragunta, Bharathi Reddy Kunduru, N.J.P. Subhashini, Synthesis, anti-diabetic evaluation and molecular docking studies of 4-(1-aryl-1H-1, 2, 3-triazol-4-yl)-1,4-dihydropyridine derivatives as novel 11-β hydroxysteroid dehydrogenase-1 (11β-HSD1) inhibitors, Bioorganic Chemistry, Volume 90, 2019, 103056, https://doi.org/10.1016/j.bioorg.2019.103056.
Anand P. Kunnumakkara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B. Aggarwal BB (September 2008). "Cancer is a preventable disease that requires major lifestyle.
Yerragunta, V., Waghray, K., Shivraj, . and Subhashini, N. J. P. (2021) “Anti-Cancer Activity of Andrographolide: A Review”, Journal of Pharmaceutical Research International, 33(43B), pp. 1–9. doi: 10.9734/jpri/2021/v33i43B32519.
Badakhshan Mahdi Pour, Sreenivasan Sasidharan, Rameshwar Naidu, Jegathambigai, and Ramanathan Surash Anti-leukemia Activity of Methanolic Extracts of Lantana camara, Ano-leukemia Activity of Methanolic Extracts of Lantana camara. 1, 5, 274- Caspases Activation. Pathol Oncol Res. 2015 Apr. 21(2)-325-31 Doi 10.1007/s12253-014-9824-4. Epub 2014 Aug 22 PMID 25145450. changes" Pharmaceutical Research. 25 (9). 2097-116. doi:10.1007/s11095-008-9661.
Gurish CS. Negit, & Subrat Sharmal & Subesh CR. Vishvakarmal & Sher S. Samami & Rakesh K. Maikhurit & Ram C. Prasadi & Lok MS Palmil Ecology and Use of Lantana amara in India. The Botanical Review (2019) 85 109-130.
Hiremath, Kavita Y Jagadeesh, Narasimhappagari Belur, Shivakumar Kulkarni, Supreeth S Inamdar, Shashikala R. A lectin with anti-microbial and anti proliferative activites from Lantana camara, a medicinal plant, 2020, Protein Expression and Purification, 170 105574.
Hublikar LV, Ganachari SV, Patil VB, Nandi 5, Honnad A. Anticancer potential of biologically synthesized silver nanoparticles using Lantana camara leaf extract. Prog Biomater 2023 Jon, 12(2): 155-169. doi:
100740204-023-00219-9. Epub 2023 Apr 24. PMID: 37093445, PMCID: PMC10154448.
DineshBhaskar, Dheyaa Shakir Mashrea, Nagarathna Amresh, Balasubramanian Sathyamurthy. Invitro studies on the effect of Lantana Camera Linn. In liver cancer HEPG cell lincs ejpmr. 2017, 4(9), 539-545.
Kocarnik, JM; others (2022) "Cancer Incidence, Mortality, Years of Life Last, Years Lived With Disability, and Disability-Adjusted Life Years for 29 Cancer Groups From 2010 to 2019. A Systematic Analysis for the Global Burden of Disease Study 2019 JAMA Oncology 8 (3) 420-444 Doi:10.1001/jamaoncol. 2021.6987. PMC 8719276. PMID 34967848.
Monika Chauhan, Zahid Ahmad Dhar, Varun Gorki, Sonia Sharma, Ashwani Koul, Shashi Bala, Ramandeep Kaur, Sukhbir Kaur, Manu Sharma, Neelima Dhingra, Exploration of anticancer potential of Lantadenes from weed Lantana camara: Synthesis, in silico, in vitro and in vivo studies, Phytochemistry, Volume 206, 2023,113525,ISSN 0031-9422.
Neha Goyal, Gyan Prakash Sharma. Lantana comara L. (senow fare) an enigmatic complex 14 apr 2015 Neo Biota 25: 15-26.
Nitin P Kurade, Vikas Jaitak, Vijay K Kaul & Om P Sharma (2010) Chemical composition and antibacterial activity of essential oils of Lantana camara, Ageratum houstonianum and Eupatorium adenophorum, Pharmaceutical Biology, 48:5, 539-544, DOI: 10.3109/13880200903193336 4. PMC 2515569. PMID 18626751.
"Global Cancer Statistics 2020 GLOBOCAN Estomates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries". CA. A Cancer Journal for Clinicians. 71 (3): 209-249 dor: 10.3322/caac: 21660. PMID 33538338 S2CID 231804598.
Rajendiran R, Balasubramani G, Raja RK, RajaM, Govindan RRR, Girija EK, Perumal P. Lantana camara Linn root extract-mediated gold nanoparticles and their in vitro antioxidant and cytotoxic potentials 017: 205/19.
Subhalakshmi Ghosh, Madhushree Das Sarma, Amarendra Patra, Barnasrı Hazra Anti-inflammatory, and anticancer compounds isolated from Ventilago madraspatana Gaertn. Rubia cordifolia Linn and Lantana camara Linn. Journal of Pharmacy and Pharmacology, Volume 62, Issue 9, September 2010, Pages 1158-1166.
Sumit Kumar Ranal, Guno SindhuChakraborthy2. Avijit Mazumderi, Saumya Dasi, Gunjan Sodhil, Kapil Kumari, Pawan Kumar A herbal medication with diverse clinical pharmacology Sumit KR volume 7 issue 2 apr-jun 2019 ISSN 2347-6567.
Sung H, Ferly J, Siegel RL. Lavers Anne M, Soerjonmataram 1, Jemal A, et al (May 2021) World Cancer Report 2014. World Health Organization 2014 pp. Chapter 52. ISBN 978-92.
Yerragunta Vishwanadham, Kavita Waghray, Shivraj, D Appajı, Molecular Docking Studies of Novel Andrographolide analogs using Phosphoinositide 3kinase (PI3K) as potent anti-cancer inhibitor, Eur. Chem. Bull 2023, 12(Special Issue 4), 19594-19607.
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
