Exercise-Induced Changes In Blood Lactate And Muscle Fatigue: A Comparative Study Between Trained And Untrained Individuals
DOI:
https://doi.org/10.63682/jns.v14i32S.8541Keywords:
Lactate, Muscle Fatigue, Exercise, TrainingAbstract
Background: Exercise induces physiological stress, primarily with elevated blood lactate and muscle fatigue during exercise, particularly anaerobic exercise. Trained respondents respond by enhanced lactate clearance and delayed fatigue development, whereas untrained respondents can be characterized by an acute accumulation of lactate. Learning about these distinctions is useful in the maximization of training programs and corporate performance in athletics and rehabilitation.
Objectives: This experiment seeks to determine the feminine differences in plasma lactate versus muscle fatigue rates in untrained subjects and those who are trained during graded exercise to determine the effect of level of training on metabolic stress performance along with recovery kinetics.
Study design: A Prospective Study.
Place and duration of study: Nishtar Medical University Multan, From May 2024 To May 2025
Methods:A graded treadmill exercise was done by twenty (10 trained and 10 untrained) participants. Baseline, peak and post-exercise blood lactate was assessed by finger-prick acivities. Muscle fatigue was measured through isometric handgrip strength and perceived exertion (RPE). A comparison in the lactate reaction of the participants and fatigue thresholds was performed by providing mean values, standard deviation, and independent t-tests, which indicated statistical significance (p < 0.05).
Results: Included applicants were recruited as a sample of 20 participants aged 20-25 years and 10 trained participants (mean 24.6 +/- 2.1 years) and 10 untrained (mean 25.2 +/- 2.4 years). It resulted in the lower peak lactate levels in trained individuals (6.1 +/- 0.8 mol/L) than in untrained individuals (8.4 +/- 1.2 mol/L, p = 0.002). The trained group developed muscle fatigue later (longer handgrip endurance times 52.3 +/- 6.7 sec.) compared to the untrained group (38.5 +/- 5.9 sec., p = 0.001). During recovery, the trained individuals had faster lactate discharge. These distinctions in food patterns imply better metabolism in the trained group.
Conclusion: Individuals trained revealed a greater capacity to utilize lactate, increased resistance to fatigue, and experienced faster recovery. These results hold the significance of the physiological advantages of regular training on lactate metabolism fatigue tolerance. These adaptations are paramount in the plan of more effective fitness and rehabilitation programs. Future studies can also examine how different types and intensity of training, and duration effect the management of lactate dynamics and fatigue limits among various populations
Downloads
Metrics
References
Nakamura K, Fujiwara T, Horsehide S, Ishiyama Y, Take M, Ozawa S, Kari K. Differences in exercise‐induced blood pressure changes between young trained and untrained individuals. The Journal of Clinical Hypertension. 2021 Apr; 23(4):843–848. https://doi.org/10.1111/jch.14162
Kush K, Matysiak J, Kook ZJ, Ciekot-Sołtysiak M, Klupczyńska-Gabryszak A, Zarębska EA, Plea S, Dereziński P, Zieliński J. Exercise-induced response of proteinogenic and non-proteinogenic plasma free amino acids is sport-specific: A comparison of sprint and endurance athletes. PLOS ONE. 2024 Aug 30; 19(8):e0309529. https://doi.org/10.1371/journal.pone.0309529
Broome SC, Pham T, Brachium AJ, Nearing R, Wang HW, Hickey AJ, Mitchell CJ, Merry TL. Matos supplementation augments acute exercise-induced increases in muscle PGC1α mRNA and improves training-induced increases in peak power independent of mitochondrial content and function in untrained middle-aged men. Redo Biology. 2022 Jul 1; 53:102341. https://doi.org/10.1016/j.redox.2022.102341
Zara R, Devrim-Lanpir A, Guazzotti S, Ali Redhead A, Prokopidis K, Spadaccini D, Cinnabar R, Cone E, Henselmans M, Aragon AA. Effect of soy protein supplementation on muscle adaptations, metabolic and antioxidant status, hormonal response, and exercise performance of active individuals and athletes: A systematic review of randomized controlled trials. Sports Medicine. 2023 Dec; 53(12):2417–2446. https://doi.org/10.1007/s40279-023-01940-9
Yan, B., Yao, S., Zhang, J., Li, C., Han, T., Hub, Q., & Live, K. (2025). Effects of asymmetric load bench press offset training on muscle activation levels and exercise-induced fatigue in collegiate bodybuilders. Frontiers in Physiology, 16, 1592477. https://doi.org/10.3389/fphys.2025.1592477
Elite, C. D., Novice, P. V., Rica, R. L., Barros, B. M., Machado, A. F., Evangelista, A. L., Elite, R. D., Braun, V. G., Maia, A. F., & Bubaline, D. S. (2023). Exercise-induced muscle damage after a high-intensity interval exercise session: Systematic review. International Journal of Environmental Study and Public Health, 20(22), 7082. https://doi.org/10.3390/ijerph20227082
Fugato, E., Prepaid, H. P., & Saguaro, D. (2024). L-carnation supplementation decreases post-exercise blood lactate levels and enhances aerobic capacity in trained individuals: Systematic review and meta-analysis. Trends in Sport Sciences, 31(3). https://doi.org/10.23829/TSS.2024.31.3
Ross, M., Karl, C. K., Ferguson, R., Gavin, T. P., & Hells ten, Y. (2023). Exercise-induced skeletal muscle angiogenesis: Impact of age, sex, angiocrines and cellular mediators. European Journal of Applied Physiology, 123(7), 1415–1432. https://doi.org/10.1007/s00421-023-05197-7
Franz, A., et al. "Comparison of Metabolic, Ionic, and Electrolyte Responses to Exhaustive Low‐Load Strength Training With and Without Blood Flow Restriction and High‐Load Resistance Training." Scandinavian Journal of Medicine & Science in Sports, vol. 34, no. 9, 2024, e14721. https://doi.org/10.1111/sms.14721
Markus, I., Constantine, K., Hoffman, J. R., Bartolommeo, S., & Genre, Y. (2021). Exercise-induced muscle damage: Mechanism, assessment and nutritional factors to accelerate recovery. European Journal of Applied Physiology, 121(4), 969–992. https://doi.org/10.1007/s00421-020-04552-3
Zara, R., Devrim-Lanpir, A., Guazzotti, S., Redhead, A. A., Prokopidis, K., Spadaccini, D., Cinnabar, R., Cone, E., Henselmans, M., & Aragon, A. A. (2023). Effect of soy protein supplementation on muscle adaptations, metabolic and antioxidant status, hormonal response, and exercise performance of active individuals and athletes: A systematic review of randomized controlled trials. Sports Medicine, 53(12), 2417–2446. https://doi.org/10.1007/s40279-023-01953-2
Ross, M., Karl, C. K., Ferguson, R., Gavin, T. P., & Hells ten, Y. (2023). Exercise-induced skeletal muscle angiogenesis: Impact of age, sex, angiocrines and cellular mediators. European Journal of Applied Physiology, 123(7), 1415–1432. https://doi.org/10.1007/s00421-023-05197-7
Schierbauer, J., Wolf, A., Wachsmuth, N. B., Massena, N., & Schmidt, W. F. (2023). Relationship between blood volume, blood lactate quantity, and lactate concentrations during exercise. Metabolites, 13(5), 632. https://doi.org/10.3390/metabo13050632
Markus, I., Constantine, K., Hoffman, J. R., Bartolommeo, S., & Genre, Y. (2021). Exercise-induced muscle damage: Mechanism, assessment and nutritional factors to accelerate recovery. European Journal of Applied Physiology, 121(4), 969–992. https://doi.org/10.1007/s00421-020-04552-3
Kowalski, T., Klusiewicz, A., Rabid, K., Walk, A., & Starczewski, M. (2024). Comparative study of different respiratory muscle training methods: Effects on cardiopulmonary indices and athletic performance in elite short-track speed skaters. Life, 14(9), 1159. https://doi.org/10.3390/life14091159
Zoo, C., Yin, Y., Zhen, Z., Mu, R., Liang, Y., Wang, S., & Ye, C. (2025). Unlocking the full potential of resistance training: A comparative analysis of low- and high-intensity effects on neurotrophic growth factors and homocysteine. Discover Applied Sciences, 7(2), 108. https://doi.org/10.1007/s42452-024-01408-x
Mons, V., Laving, C., Mesta, O., Mauro, B., & Blain, G. M. (2025). Ageing exacerbates the adverse effects of respiratory muscle fatigue on vascular function, locomotors muscle fatigue and exercise performance in males. Experimental Physiology. Advance online publication. https://doi.org/10.1113/EP092422
Rosa, A., Coleman, M., Haunt, C., Grid, J., & Schoenfeld, B. J. (2023). Repetition performance, rating of perceived discomfort, and blood lactate responses to different rest interval lengths in single-joint and multipoint lower-body exercise. The Journal of Strength & Conditioning Study, 37(7), 1350–1357. https://doi.org/10.1519/JSC.0000000000004437
Downloads
Published
How to Cite
Issue
Section
License

This work is licensed under a Creative Commons Attribution 4.0 International License.
You are free to:
- Share — copy and redistribute the material in any medium or format
- Adapt — remix, transform, and build upon the material for any purpose, even commercially.
Terms:
- Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
- No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.