A Comparative Study An In-Vitro Anti-Urolithiasis Activity Of Hydro-Alcoholic Seed Extract: Glycine Max L. And Achyranthes Aspera L.

Abstract

Glycine max linn, commonly referred to as soybeans, have been researched for their possible advantages in treating urolithiasis. The seeds contain chemicals, such as oxalate-degrading enzymes and phytate that can inhibit the production of kidney stones. These substances are abundant in isoflavone, flavonoid, anthocyanidin phyto-constituents, which have the ability to decrease inflammation and oxidative stress in the urinary system. Achyranthes aspera linn seeds has diuretic and anti-inflammatory characteristics, making them beneficial in traditional medicine. Research has demonstrated their ability to dissolve kidney stones, improve kidney functions, and alleviate discomfort associated with urolithiasis. The current study aimed to assess the effectiveness of both plants in laboratory methods for urolithiasis by using a crystal nucleation assay at dosages of 0.1, 1 mg, and 10 mg/ml, respectively. Glycine max linn exhibited a notable increase in the rate of crystal nucleation compared to Achyranthes aspera linn. This implies that Glycine max may have a stronger ability to promote crystal formation in a solution. The cause of this phenomenon can be described presence of bio compounds in Glycine max, which perhaps includes substances that improve nucleation processes. A Fourier Transform Infrared (FT-IR) analysis was conducted on 10-50 mg of calcium oxalate (CaOx) crystals generated during the crystal nucleation experiment. The analysis confirmed the existence within the crystal structure of calcium and oxalate ions. The results of the inquiry showed that Glycine max exhibited the highest level of crystal aggregation (56.69 %) at a concentration of 1000 mg/ml, but Sample Achyranthes aspera demonstrated the most crystal aggregation (79.26 %) at the same concentration. Calcium CaOX aggregation was most significantly inhibited by A. aspera which was very close to Cystone (80.79 %). The study examined the impact of GM extract and AA at concentrations of 0.1, 1 and 10 mg/ml on the Nucleation assay. the dimensions of the calcium oxide stone were measured using this approach and treated with AA extract at varying concentrations for varying lengths of time and GM extract also with different time intervals, when compared to a typical medication cystone. According to the Nucleation assay's results on anti-urolithiatic activity, after 270 minutes, AA extract showed a higher percentage of inhibition at a dosage of 10 mg/ml, in contrast to the prescribed medication cystone tablets However, 0.1 mg/ml of GM extract for 240 minutes, exhibited a significant % inhibition when compared to standard drug cystone. The study showed that the Achyranthes aspera linn seed extract shown a potent antiurolithiatic effect on crystal aggregation and on nucleation assay when compared to another selected seed extract of Glycine max Linn, the treatment of renal stone disease may be derived from this.