Extraction, Phytochemical Analysis and Study of TPC and TFC In Extract of Achyranthes ASPERA

Authors

  • Manjusha Shandilya
  • Akhlesh Kumar Singhai

Keywords:

Achyranthes aspera, phytochemical screening, total phenolic content, total flavonoid content, antioxidant activity, methanolic extract

Abstract

The current study investigates the extraction, phytochemical screening, and assessment of total phenolic and flavonoid content in different solvent extracts of Achyranthes aspera aerial parts. Various extracts, including chloroform, ethyl acetate, methanolic, and aqueous extracts, were prepared, and their yields were calculated. Phytochemical analysis revealed the presence of important bioactive compounds such as alkaloids, flavonoids, diterpenes, phenols, proteins, saponins, and tannins, with methanolic extract showing the highest yield and the most significant phenolic and flavonoid content. The methanolic extract exhibited the highest total phenolic content (1.63 mg/100 mg) and flavonoid content (1.45 mg/100 mg), suggesting its potent antioxidant activity. The findings support the therapeutic potential of Achyranthes aspera in combating oxidative stress and liver diseases. These results lay the groundwork for further studies on the pharmacological properties of Achyranthes aspera and its bioactive constituents.

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References

Sarvaiya C. Isolation and Characterization of Bioactives from Roots of Achyranthes Asperal with Special Reference to Anti-Appetite and Anti-Hyperlipidemic Activity (Doctoral dissertation, Institute of Pharmacy, Nirma University, A'bad).

Rigat M, Vallès J, Iglésias J, Garnatje T. Traditional and alternative natural therapeutic products used in the treatment of respiratory tract infectious diseases in the eastern Catalan Pyrenees (Iberian Peninsula). Journal of Ethnopharmacology. 2013 Jul 9;148(2):411-22.

Nunes CD, Barreto Arantes M, Menezes de Faria Pereira S, Leandro da Cruz L, de Souza Passos M, Pereira de Moraes L, Vieira IJ, Barros de Oliveira D. Plants as sources of anti-inflammatory agents. Molecules. 2020 Aug 15;25(16):3726.

Vishwakarma M, Patel A, Jain D. A review of current research on traditional medicines for the treatment of gastrointestinal and biliary disorders. Current Traditional Medicine. 2024 Jun 1;10(3):129-48.

Lam P, Cheung F, Tan HY, Wang N, Yuen MF, Feng Y. Hepatoprotective effects of Chinese medicinal herbs: a focus on anti-inflammatory and anti-oxidative activities. International Journal of Molecular Sciences. 2016 Mar 29;17(4):465.

Murugan K, Sekar K, Sangeetha S, Ranjitha S, Sohaibani SA. Antibiofilm and quorum sensing inhibitory activity of Achyranthes aspera on cariogenic Streptococcus mutans: An in vitro and in silico study. Pharmaceutical biology. 2013 Jun 1;51(6):728-36.

Al-Rimawi F, Rishmawi S, Ariqat SH, Khalid MF, Warad I, Salah Z. Anticancer activity, antioxidant activity, and phenolic and flavonoids content of wild Tragopogon porrifolius plant extracts. Evidence‐Based Complementary and Alternative Medicine. 2016;2016(1):9612490.

Subramanya SB, Venkataraman B, Meeran MF, Goyal SN, Patil CR, Ojha S. Therapeutic potential of plants and plant derived phytochemicals against acetaminophen-induced liver injury. International journal of molecular sciences. 2018 Nov 28;19(12):3776.

Nikhal SB, Dambe PA, Ghongade DB, Goupale DC. Hydroalcoholic extraction of Mangifera indica (leaves) by Soxhletion. International Journal of Pharmaceutical Sciences 2010; 2 (1): 30-32.

Prashant Tiwari, Bimlesh Kumar, Mandeep Kaur, Gurpreet Kaur, Harleen Kaur. Phytochemical screening and Extraction: A Review. Int. Pharmaceutica. Sciencia. 2011, 1 (1), 98-106.

Geeta Parkhe, Deepak Bharti. Phytochemical investigation and determination of total phenols and flavonoid concentration in leaves extract of Vitex trifolia Linn. Journal of drug delivery & therapeutics. 2019; 9(4-A):705-707.

Meda, A., Lamien, C.E., Romito, M., Millogo, J. and Nacoulma, O.G. (2005). Determination of the total phenolic, flavonoid and proline contents in Burkina Fasan honey, as well as their radical scavenging activity. Food Chemistry, 91: 571-577.

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Published

2025-08-13

How to Cite

1.
Shandilya M, Singhai AK. Extraction, Phytochemical Analysis and Study of TPC and TFC In Extract of Achyranthes ASPERA. J Neonatal Surg [Internet]. 2025Aug.13 [cited 2025Sep.19];14(32S):7496-503. Available from: https://www.jneonatalsurg.com/index.php/jns/article/view/8871