Formulation and Evaluation of Cubosomal In-Situ Gel for Topical Aaplication

Authors

  • Kardile Gangasagar B.
  • Shivappa N. Nagoba
  • Shital N. Humnabade
  • Sneha C. Kakade

DOI:

https://doi.org/10.63682/jns.v14i32S.9199

Keywords:

Thin film hydration method, Cubosome, Thermo-sensititve, Cold method, Topical application

Abstract

Background: Nanotechnology has emerged as a transformative approach in drug delivery, particularly for enhancing the therapeutic efficacy and targeting of poorly soluble drugs.The present study focuses on the formulation and evaluation of a thermosensitive cubosomal in-situ gel for the vaginal delivery of voriconazole, aimed at enhancing local antifungal therapy. Methods: Cubosomes were prepared using the thin film hydration method incorporating voriconazole, poloxamer 188, GMO and a suitable solvent, followed by hydration with distilled water to form a stable cubosomal dispersion. The dispersion was further integrated into an in-situ gel system using thermosensitive polymers Poloxamer 407 and Carbopol 940P prepared under cold conditions (2–8°C) to prevent premature gelation.

Results: Among the formulations, VF5 exhibited superior cubosomal characteristics with a drug content of 78.88%, entrapment efficiency of 73.24% and in-vitrodrug diffusion 77.72% at 12 hours. Particle size analysis showed a Z-average of 102.0nm, PDI at 0.381 and Zeta potential at -10.2 mV, indicating good stability. Upon incorporation into the in-situ gel, formulation V2 demonstrated optimal physicochemical properties, including a pH at 5.4, Viscosity at 1128.33±12.89 cps and excellent spreadability. Gelation temperature was found to be 36±0.30°C with strong gel strength (+++) and a gelation time of 11seconds. V2 also showed the highest Drug Content (81.84%), Entrapment Efficiency (73.93%) and Drug Diffusion (79.57% at 12hrs) highlighting its potential as an effective topical drug delivery system.

Conclusion: These findings suggest that the cubosomalin-situ gel system offers a promising platform for controlled and targeted delivery of antifungal agents.

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References

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Published

2025-09-23

How to Cite

1.
Gangasagar B. K, N. Nagoba S, N. Humnabade S, C. Kakade S. Formulation and Evaluation of Cubosomal In-Situ Gel for Topical Aaplication. J Neonatal Surg [Internet]. 2025Sep.23 [cited 2025Oct.1];14(32S):8546-54. Available from: https://www.jneonatalsurg.com/index.php/jns/article/view/9199