Antioxidant And Anticancer Activity of Praecitrullus Fistulosus Fruit Extracts on Mcf-7 Cancer Cell Lines

Authors

  • Vignesh Annam, Rajesh Sharma
  • Nidhi Tyagi
  • Nitin kumar

Keywords:

Praecitrullus fistulosus, Praecitrullus fistulosus, Antioxidant activity, Antioxidant activity, DPPH assay, DPPH assay, Anticancer activity, Anticancer activity, MCF-7 cell line, MCF-7 cell line, SRB assay, SRB assay, Polyphenols, Polyphenols, Flavonoids, Flavonoids, Herbal medicine, Herbal medicine

Abstract

Objective: This study aims to evaluate the antioxidant and anticancer potential of Praecitrullus fistulosus fruit extracts, particularly their effectiveness against the MCF-7 human breast cancer cell line.

Methods: Fresh fruits of Praecitrullus fistulosus were collected, authenticated, and extracted using sequential Soxhlet extraction with different solvents (petroleum ether, chloroform, ethyl acetate, acetone, and ethanol). The antioxidant activity was assessed using the DPPH free radical scavenging assay, while the anticancer activity was evaluated against the MCF-7 breast cancer cell line using the Sulforhodamine B (SRB) assay.

Results: The ethanolic extract exhibited the highest DPPH scavenging activity (63.157%), demonstrating strong antioxidant potential. The cytotoxicity assessment showed a dose-dependent inhibition of MCF-7 cells, with the ethanolic extract displaying the highest anticancer activity (50.71% inhibition at 80 µg/ml). The cytotoxic effect of the extracts was attributed to their rich content of polyphenols, flavonoids, and saponins, which are known to induce apoptosis and inhibit cancer cell proliferation.

Conclusion: The results suggest that Praecitrullus fistulosus fruit extracts, particularly the ethanolic extract, possess significant antioxidant and anticancer activities. These findings highlight the plant’s potential as a natural source of bioactive compounds for cancer therapy. Further studies are required to isolate and characterize the active compounds responsible for these effects.

Downloads

Download data is not yet available.

References

Bray F., Ferlay J., Soerjomataram I., Siegel R.L., Torre L.A., Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 2018;68:394–424. doi: 10.3322/caac.21492. [DOI] [PubMed] [Google Scholar]

Karpuz M., Silindir-Gunay M., Ozer A.Y. Current and Future Approaches for Effective Cancer Imaging and

Treatment. Cancer Biother. Radiopharm. 2018;33:39–51. doi: 10.1089/cbr.2017.2378. [DOI] [PubMed] [Google Scholar]

Nobili S., Lippi D., Witort E., Donnini M., Bausi L., Mini E., Capaccioli S. Natural compounds for cancer treatment and prevention. Pharmacol. Res. 2009;59:365–378. doi: 10.1016/j.phrs.2009.01.017. [DOI] [PubMed] [Google Scholar]

Cheng H. Advanced Textbook on Traditional Chinese Medicine and Pharmacology. New World Press; Beijing, China: 1995.

Shehab N.G., Mahdy A., Khan S.A., Noureddin S.M. Chemical constituents and biological activities of Fagonia indica Burm F. Res. J. Med. Plant. 2011;5:531–546. doi: 10.3923/rjmp.2011.531.546. [DOI] [Google Scholar]

Faried A., Kurnia D., Faried L., Usman N., Miyazaki T., Kato H., Kuwano H. Anticancer effects of gallic acid isolated from Indonesian herbal medicine, Phaleria macrocarpa (Scheff.) Boerl, on human cancer cell lines. Int. J. Oncol. 2007;30:605–613. doi: 10.3892/ijo.30.3.605. [DOI] [PubMed] [Google Scholar]

Sohi K.K., Mittal N., Hundal M.K., Khanduja K.L. Gallic acid, an antioxidant, exhibits antiapoptotic potential in normal human lymphocytes: A Bcl-2 independent mechanism. J. Nutr. Sci. Vitaminol. (Tokyo) 2003;49:221– 227. doi: 10.3177/jnsv.49.221. [DOI] [PubMed] [Google Scholar]

Inoue M., Suzuki R., Koide T., Sakaguchi N., Ogihara Y., Yabu Y. Antioxidant, gallic acid, induces apoptosis in HL-60RG cells. Biochem. Biophys. Res. Commun. 1994;204:898–904. doi: 10.1006/bbrc.1994.2544. [DOI] [PubMed] [Google Scholar]

Sun J., Chu Y.-F., Wu X., Liu R.H. Antioxidant and antiproliferative activities of common fruits. J. Agric. Food Chem. 2002;50:7449–7454. doi: 10.1021/jf0207530. [DOI] [PubMed] [Google Scholar]

Taraphdar A.K., Roy M., Bhattacharya R. Natural products as inducers of apoptosis: Implication for cancer therapy and prevention. Curr. Sci. 2001:1387–1396. [Google Scholar].

Downloads

Published

2025-05-04

How to Cite

1.
Rajesh Sharma VA, Tyagi N, kumar N. Antioxidant And Anticancer Activity of Praecitrullus Fistulosus Fruit Extracts on Mcf-7 Cancer Cell Lines. J Neonatal Surg [Internet]. 2025May4 [cited 2025Nov.13];14(5S):1039-50. Available from: https://www.jneonatalsurg.com/index.php/jns/article/view/9537

Issue

Vol. 14 No. 5S (2025): Journal of Neonatal Surgery

Section

Original Article

License

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

You are free to:

  • Adapt — remix, transform, and build upon the material for any purpose, even commercially.

Terms:

  • Attribution — You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • No additional restrictions — You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.