Antimicrobial Efficacy of Iron Oxide Nanoparticles Incorporated in Commercial Toothpaste Against Streptococcus mutans, Enterococcus faecalis, Candida albicans, and Lactobacillus
DOI:
https://doi.org/10.52783/jns.v14.1667Keywords:
Iron Oxide Nanoparticles, Antimicrobial Toothpaste, Biofilm Inhibition, Dental Caries PreventionAbstract
Background: Maintaining oral hygiene is Crucial for preventing dental diseases associated with microbial biofilms. Traditional toothpaste formulations have limitations in effectively combating biofilm-associated pathogens such as Streptococcus mutans, Enterococcus faecalis, Candida albicans, and Lactobacillus. Iron oxide nanoparticles have shown promise in enhancing antimicrobial efficacy due to their ability to generate reactive oxygen species (ROS) and disrupt microbial cell walls.
Aim: To evaluate the antimicrobial efficacy of iron oxide-enriched toothpaste against key oral pathogens to determine its potential in improving oral health and biofilm inhibition.
Materials and Methods: Iron oxide nanoparticles were synthesized and incorporated into a commercial toothpaste at varying concentrations (25 µg/mL, 50 µg/mL, and 100 µg/mL). The antimicrobial activity was avaluated using agar well diffusion, time-kill curve, and biofilm inhibition assays. The study analyzed microbial inhibition against S. mutans, E. faecalis, C. albicans, and Lactobacillus using statistical methods, including one-way ANOVA, Tukey HSD post hoc tests, independent t-tests, effect size calculations (Cohen’s d, Hedges' correction, and Glass’s delta), and multivariate analysis (Pillai’s Trace, Wilks’ Lambda, Hotelling’s Trace, and Roy’s Largest Root).
Results: Iron oxide nanoparticle-enriched toothpaste demonstrated significant antimicrobial activity, with a dose-dependent reduction in microbial growth. The highest inhibition was observed for S. mutans, followed by E. faecalis, C. albicans, and Lactobacillus. The 100 µg/mL Fe₂O₃ NP formulation showed the most pronounced inhibition, significantly outperforming commercial toothpaste.
Conclusion: The findings indicate that Iron oxide nanoparticle-enriched toothpaste effectively inhibits biofilm formation and microbial growth, particularly against S. mutans. This suggests its potential as an advanced oral hygiene product to combat dental caries and biofilm-associated infections. Further research is needed to assess its long-term safety and efficacy in clinical settings.
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References
Schwiertz A. Microbiota of the Human Body: Implications in Health and Disease. Springer; 2016. 160 p.
Dental Caries: Diagnosis, Prevention and Management. BoD – Books on Demand; 2018. 178 p.
Karabiber E, Ilki A, Gökdemir Y, Vatansever HM, Olgun Yıldızeli Ş, Ozen A. Microbial Isolates and Antimicrobial Resistance Patterns in Adults with Inborn Errors of Immunity: A Retrospective
Amer Assoc of Public Health Dentistry. Burt and Eklund’s Dentistry, Dental Practice, and the Community - E-Book: Burt and Eklund's Dentistry, Dental Practice, and the Community - E-Book. Elsevier Health Sciences; 2020. 354 p.Longitudinal Analysis of Sputum Cultures. Int Arch Allergy Immunol. 2024 Oct 21;1–12.
Iron oxide nanoparticles in biological systems: Antibacterial and toxicology perspective. JCIS Open. 2021 Dec 1;4:100027.
Gs S, Sarvathikari R, Amhr Alkandari A, Sudhamani, Nawaz MKK, L J, et al. Prevalence of Incidental Findings and Assessment of Maxillary Sinus Pathologies and Dental Diseases Using Cone-Beam Computed Tomography (CBCT) in the Tamil Nadu Population: A Retrospective Study. Cureus. 2024 Sep;16(9):e68929.
de Lima JM, Bonan PR, da Cruz Perez DE, Hier M, Alaoui-Jamali MA, da Silva SD. Nanoparticle-Based Chemotherapy Formulations for Head and Neck Cancer: A Systematic Review and Perspectives. Nanomaterials (Basel) [Internet]. 2020 Sep 29;10(10). Available from: http://dx.doi.org/10.3390/nano10101938
Kujan O, Mello FW, Warnakulasuriya S. Malignant transformation of oral submucous fibrosis: A systematic review and meta-analysis. Oral Dis. 2021 Nov;27(8):1936–46.
Ghorbanizadeh S, Karami F, Delfani S, Shakibaie M, Razlansari A, Rezaei F. Antibacterial effects and cellular mechanisms of iron oxide magnetic nanoparticles coated by piroctone olamine against some cariogenic bacteria. Ann Med Surg (Lond). 2022 Sep;81:104291.
Kishen A. Nanotechnology in Endodontics: Current and Potential Clinical Applications. Springer; 2015. 206 p.
Naha PC, Liu Y, Hwang G, Huang Y, Gubara S, Jonnakuti V, et al. Dextran-Coated Iron Oxide Nanoparticles as Biomimetic Catalysts for Localized and pH-Activated Biofilm Disruption. ACS Nano. 2019 May 28;13(5):4960–71.
Ahmad T, Phul R, Khatoon N, Sardar M. Antibacterial efficacy of Ocimum sanctum leaf extract-treated iron oxide nanoparticles. New J Chem. 2017 Feb 28;41(5):2055–61.
Shahid H, Shah AA, Shah Bukhari SNU, Naqvi AZ, Arooj I, Javeed M, et al. Synthesis, Characterization, and Biological Properties of Iron Oxide Nanoparticles Synthesized from Honey. Molecules [Internet]. 2023 Sep 7;28(18). Available from: http://dx.doi.org/10.3390/molecules28186504
Kunjan F, Shanmugam R, Govindharaj S. Evaluation of Free Radical Scavenging and Antimicrobial Activity of Coleus amboinicus-Mediated Iron Oxide Nanoparticles. Cureus. 2024 Mar;16(3):e55472.
Pillai SC, Lang Y. Toxicity of Nanomaterials: Environmental and Healthcare Applications. CRC Press; 2021. 246 p.
Lakshmanan L, Gurunathan D, Shanmugam R. Effectiveness of white tea-mediated silver nanoparticles as an intracanal irrigant against Enterococcus faecalis: An in vitro study. Dent Med Probl. 2024 Jul-Aug;61(4):593–8.
Batool M, Khurshid S, Qureshi Z, Hassan A, Siddique MBA, Naveed S, et al. Study of biogenically fabricated transition metal oxides nanoparticles on oral cavity infectious microbial strains. Inorganic and Nano-Metal Chemistry [Internet]. 2021 Jun 3 [cited 2025 Jan 31]; Available from: https://www.tandfonline.com/doi/abs/10.1080/24701556.2020.1811729
Ahmed ME, Abdul Muhsin ZA. Synergistic Effect of Gentamicin and Iron Oxide Nanoparticles on phzM Gene of Pseudomonas aeruginosa. Mikrobiol Z. 2024 Jun 22;86(3):27–39.
Tasnim NT, Ferdous N, Rumon MMH, Shakil MS. The Promise of Metal-Doped Iron Oxide Nanoparticles as Antimicrobial Agent. ACS Omega. 2024 Jan 9;9(1):16–32.
Caldeirão ACM, Araujo HC, Tomasella CM, Sampaio C, dos Santos Oliveira MJ, Ramage G, et al. Effects of Antifungal Carriers Based on Chitosan-Coated Iron Oxide Nanoparticles on Microcosm Biofilms. Antibiotics. 2021 May 17;10(5):588.
Makhlouf ASH, Barhoum A. Fundamentals of Nanoparticles: Classifications, Synthesis Methods, Properties and Characterization. William Andrew; 2018. 668 p.
Pandiyan I, Arumugham MI, Doraikannan SS, Rathinavelu PK, Prabakar J, Rajeshkumar S. Antimicrobial and Cytotoxic Activity of and -Mediated Silver Nanoparticles - An Study. Contemp Clin Dent. 2023 Apr-Jun;14(2):109–14.
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