Antimicrobial Potential of Calcium Nanoparticles in Fluoridated Toothpaste Against Lactobacillus and Candida albicans: An In Vitro Analysis
DOI:
https://doi.org/10.52783/jns.v14.1816Keywords:
Calcium Compounds, Fluorides, Candida albicans, LactobacillusAbstract
Background: Nanotechnology has emerged as a promising field in dentistry, particularly for improving antimicrobial efficacy in oral healthcare products. Candida albicans and Lactobacillus are key microorganisms associated with early childhood caries and oral infections. While fluoride-based toothpaste is effective against cariogenic bacteria, its efficacy against Candida albicans remains limited. Incorporating calcium nanoparticles into toothpaste formulations may enhance antimicrobial properties and offer broader-spectrum protection.
Aim: To evaluate the antimicrobial efficacy of calcium nanoparticles combined with commercially available fluoride toothpaste against Lactobacillus and Candida albicans through an in vitro analysis.
Materials and Methods: Calcium oxide (CaO) nanoparticles were synthesized using a microwave irradiation method. The antimicrobial efficacy of calcium nanoparticles in combination with fluoride toothpaste (Pediflor) was assessed against Lactobacillus and Candida albicans using the agar well diffusion method. Zones of inhibition were measured, and statistical analysis was performed using one-way ANOVA to determine significant differences across different concentrations.
Results: The combination of calcium nanoparticles and fluoride toothpaste demonstrated a dose-dependent increase in antimicrobial activity, particularly against Candida albicans. However, Pediflor alone exhibited greater inhibition against Lactobacillus. Statistical analysis revealed significant differences at higher concentrations, highlighting the differential susceptibility of the microorganisms.
Conclusion: Calcium nanoparticles enhance the antifungal efficacy of fluoride toothpaste, making them a potential additive in oral care formulations. However, their antibacterial activity against Lactobacillus was lower than that of standard fluoride toothpaste. Further research is needed to optimize formulations and assess long-term effects on oral microbiota.
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