Isolation, screening and Identification of Indole Acetic Acid and Gibberellic Acid Producing Fungi from Strawberry Rhizosphere Soil
Keywords:
Rhizosphere microorganisms, Indole acetic acid, Gibberellic acid (GA3), Aspergillus spp, Fusarium spp.Abstract
The rhizosphere, a dynamic interface between plant roots and soil, hosts a diverse community of microorganisms essential to plant growth and development. Among these, rhizospheric bacteria and fungi play critical roles in nutrient cycling, pathogen suppression, and stress tolerance, which are vital for sustainable agriculture. Natural bioresources like rhizospheric microorganisms are increasingly being explored to enhance plant growth and productivity. Recent studies have focused on fungal isolates from the rhizospheric soil of strawberry plants in Bhilar, Mahabaleshwar, assessing their potential to synthesize the plant growth-promoting hormones indole acetic acid (IAA) and gibberellic acid (GA3). These hormones are instrumental in plant development, with broad applications in agriculture and horticulture. This study aimed to isolate, screen, and characterize fungal strains capable of producing IAA and GA3. Five fungal isolates were obtained from rhizospheric soil samples of strawberry varieties Chandler, Camarosa, and Nabila. The isolates were cultured in Potato Dextrose Broth to produce IAA and GA3, with an additional Potato Dextrose Agar medium enriched with L-tryptophan specifically for IAA production. Quantitative analysis using spectrophotometry revealed that isolate F67 Fusarium spp. produced the highest level of IAA (6.0734 µg/mL), while isolate F62 Aspergillus spp. yielded the highest GA3 (75.6406 µg/mL). These findings indicate that the fungal isolates from strawberry rhizospheres could serve as valuable bioresources for natural plant hormone production, supporting sustainable agriculture and horticulture practices.
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