Isolation and Characterization of Alkaline Serine Protease Producing Microorganisms from Soil

Authors

  • Abhay Ghatage
  • Rutuja Khabale
  • G. R Pathade

Keywords:

Protease, soil, enzyme, Bacillus spp, Pseudomonas spp, Aspergillus spp, Micrococcus spp

Abstract

Protease is most important group of enzymes. Protease enzyme breaks protein into smaller peptides or amino acids. They are essential for many biological processes; they help in digestion and catabolism of protein. Protease producing bacteria & molds were isolated from soil sample.Soil sample was enriched in milk broth & spread on plates of milk agar & incubated at 300 C for 48 h.The isolates obtained were Bacillus spp , Pseudomonas spp , Micrococcus  spp , and Aspergillus niger .They were characterized on the basis of morphological, cultural & biochemical properties. The screening for promising isolates on same media & another basis of selection ratio (size of zone of clearance in mm divided by area of growth of organism in mm) the best promising isolates were Bacillus spp Pseudomonas spp , Micrococcus  spp , and Aspergillus niger . Further optimization study is in progress. Protease producing microorganisms produced the clear zone around the colony. Protease are used in various industries such as food and dairy and extensively in the pharmaceutical industries for preparation of medicines

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References

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Published

2025-05-16

How to Cite

1.
Ghatage A, Khabale R, Pathade GR. Isolation and Characterization of Alkaline Serine Protease Producing Microorganisms from Soil. J Neonatal Surg [Internet]. 2025May16 [cited 2025Oct.3];14(24S):653-6. Available from: https://www.jneonatalsurg.com/index.php/jns/article/view/6023

Issue

Vol. 14 No. 24S (2025): Journal of Neonatal Surgery

Section

Original Article

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