Testing the GI50 of human lung cancer cells (A-549) with Sulforhodamine B (SRB) assay method using ethanolic extracts of Chromolaena odorata and Capparis moonii leaves
Keywords:
A-549 human lung cancer cell line, Sulforhodamine B (SRB) assay, Chromolaena odorata, Capparis moonii, GI50 inhibition, anticancerAbstract
The study investigates the in-vitro anticancer potential of ethanolic extracts from the leaves of Chromolaena odorata and Capparis moonii using the Sulforhodamine B (SRB) assay on the lung cancer cell line (A-549). The extracts of selected plants' leaves were prepared using the Soxhlet apparatus technique using ethanol as a solvent. These plants were thought to have strong anti-cancer effects because they were abundant in phytochemicals like flavonoids and phenolic compounds. Alkaloids, flavonoids, and phenolic substances were present in the extracts. Chromolaena odorata leaves extract had a GI50 of 44 µg/ml for lung cancer cell lines, whereas Capparis moonii leaves extract had a GI50 of over 80 µg/ml. When both extracts are combined, the GI50 concentration is greater than 80 µg/ml. Given the presence of phenolic as well as flavonoid components in these plants, improving their healing qualities and lowering the GI50 concentration to the appropriate level need either optimizing experimental settings or converting extracts into novel drug delivery methods.
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