Microbiological Evaluation Of Osteomyelitis With Special Reference To Antibiotic Sensitivity Pattern Of Isolates: Insights From Clinical Isolates From A Tertiary Care Hospital
DOI:
https://doi.org/10.63682/jns.v14i4.8876Keywords:
MICROBIOLOGICAL, OSTEOMYELITIS, ANTIBIOTIC SENSITIVITY PATTERN, ESBL, MBL, CLSIAbstract
Background: Osteomyelitis often develops when a bone injury becomes exposed to germs, with open wounds accounting for nearly 80% of cases. Diagnosing the condition is complex and requires a combination of clinical assessment, laboratory tests for infection markers, and radiological imaging. This study focuses on identifying the aerobic bacterial isolates associated with osteomyelitis, evaluating their antibiotic susceptibility patterns, and analyzing their resistance profiles within the community.
Aim and Objective: To study the microbiological evaluation of osteomyelitis with special reference to antibiotic sensitivity pattern of isolates from a tertiary care hospital.
Material&Methods: All clinically diagnosed Osteomyelitis samples like pus,swabs,synovial fluid,bone sequestrum,was collected under aseptic precautions. After receiving sample, it was immediately processed for culture and sensitivity according to CLSI guidelines.
Results: Out of 72 cases of osteomyelitis, 50 samples (69.4%) were culture positive, with a clear male predominance observed. Among these 50 culture-positive cases, the majority—33 patients (66%)—belonged to the age group of 21 to 50 years. The most commonly isolated organism was Staphylococcus aureus, found in 14 cases (28%), followed by Klebsiella pneumoniae in 10 cases (20%), Enterococcus species in 8 cases (16%), coagulase-negative Staphylococcus (CoNS) in 7 cases (14%), Pseudomonas aeruginosa in 6 cases (12%), Escherichia coli in 3 cases (6%), and Proteus vulgaris in 2 cases (4%). Antibiotic resistance profiling revealed that 9 isolates (18%) were methicillin-resistant Staphylococcus aureus (MRSA), and 5 isolates (10%) were methicillin-resistant coagulase-negative Staphylococcus (MRCoNS). Additionally, 3 isolates (6%) of Enterococcus showed resistance to aminoglycosides. Extended-spectrum beta-lactamase (ESBL) production was seen in 3 isolates (6%), while 4 isolates (8%) showed combined ESBL and metallo-beta-lactamase(MBL) resistance. Two isolates (4%) exhibited both ESBL and AmpC resistance, and 4 isolates (8%) demonstrated resistance to ESBL, MBL, and Amp C enzymes simultaneously.
Conclusion:Careful,Appropriate and timely care is needed to prevent osteomyelitis.MRSA screening of patients is mandatory before any elective surgical procedure to reduce cross transmission of infections.
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