Molecular Characterization Of Mdr1 Gene Inassociation To Antifungal Resistance In Pathogenic Candida Species
DOI:
https://doi.org/10.52783/jns.v14.2785Keywords:
Molecular Characterization, Virulence factors, Fluconazole, DNA, PCR, MDR1, Gene ExpressionAbstract
Introduction: A very common illness that poses serious health hazards, particularly to immunocompromised people, is candidiasis. More than a billion infections occur each year, making it the most prevalent opportunistic fungal infection affecting human health worldwide. Numerous antifungal drugs have caused pathogenic Candida species to develop both acquired and innate resistance.
Aim And Objective: To study the molecular characterization of MDR1 genein association to its antifungal resistance in pathogenic candida species
Material And Methods: This was a cross sectional study conducted in the Department of Microbiology at a tertiary care centre. A total of 962clinical samples were screened. The Culture identification, specification, Antifungal Susceptibility testing was performed according to the CLSI guidelines. The DNA was extracted using the Qiagen DNA extraction kit and the resistant gene MDR1 was detected using the PCR.
Results: In the present studyout of 962clinical samples, 51.1% (492) were culture positive, among them 28% (138) were Candida isolates. Out of which 53(38.4%) were Candida albicans while 85(61.6%) wereNon-albicans Candida. Among Non-albicans Candida,the frequency of Candida tropicalis was observed to be maximum with urine samples and least for ET secreation. It was observed that the maximum sensitivity was observed with Amphotericin-B (95%), followed by Voriconazole(85%) and itraconazole (49.2%).The prevalence of MDR1 expression was 5.7% among Candida spp.
Conclusion: Characterizing the resistance genes in Candida species isolated from a range of clinical specimens will help us better understand the pathophysiology and clinical outcomes of candidiasis.
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