Chemical constituents of Brassica juncea seed oil and its larvicidal activity against the malaria vector of Anopheles stephensi (Diptera: Culicidae)
Keywords:
Anopheles stephensi, Brassica juncea, GC-MS, FTIR, Larvicidal activity and Mustard seed oil extractAbstract
Chemical constituents of Brassica juncea (Mustard) seed oil and larvicidal activity against the malarial vector of Anopheles stephensi (Diptera: Culicidae) was studied. The essential oil was extracted by using clevenger apparatus and analyzed for chemical constituents by GC-MS and FTIR. Larvicidal activity against An. stephensi was recorded 24 hours after exposure. The LC50 and LC90 values for mosquito larvae were calculated by profit analysis. The GC-MS analysis showed that the essential oil contained 6 different components, which were identified as 2,3-methyl-tetracosanoic acid (12.47%), 2,4,6-cycloheptatrien-1-one, 3,5... (12.75%), octasiloxane, 1,1,3,3,5,7,7,9 (8.34%), Indole-2-one, 2,3-dihydro-N-hyde... (13.54%), silane, trimethyl [5-methyl-2-(1-... (20.99%), and 1,5-methyl-2-phenylindolizine(10.52%). The resonance assignment, intensity and wave number of the main peak were obtained from the absorption spectrum. Various functional groups such as alcohol, ester, carboxylic acid, halo compounds, alkenes etc. were identified by FTIR. The essential oil obtained from the seeds of B. juncea indicates good toxicity to An. stephensi with LC50 and LC90 values of 66.97 ppm and 78. 60 ppm, respectively. It also showed significant larvicidal activity against An. stephensi. It is helpful in creating practical, affordable, eco-friendly, and region-specific methods to control the malaria vector of An. stephensi.
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Anupam G, Nandita C, Goutam C. (2012) Plant extracts as potential mosquito larvicides Indian J Med Res. 135(5): 581–598.
World Health Organization (2014) World Malaria Report. WHO, Geneva.
Senthilkumar A, Verma P, Gurusubamanian G. (2009) Larvicidal and adulticidal activities of some medicinal plants against the malarial vector Anopheles stephensi (Liston). Parasitol Res. 104: 237–244.
Intirach J, Choochote W, Junkum A, Chaithong U, Champakaew D, Tuetun B, Jitpakdi A, Pitasawat B. (2012) Chemical constituents and combined larvicidal effects of selected essential oils against Anopheles cracens (Diptera: Culicidae) Psyche. 2012: 1–11.
Dharmagadda VSS, Naik SN, Mittal PK, Vasudevan P. (2005) Larvicidal activity of Tagetes patula essential oil against three mosquito species. Bio resour Technol. 96: 1235–1240.
Rutledge CR, Clarke F, Curtis A, Sackett S. (2003) Larval mosquito control, Technical bulletin of the Florida mosquito control association. 4: 16–19.
Mittal PK, Wijeyaratne P, Pandey S. (2004) Status of insecticide resistance of malaria, kala-azar and Japanese encephalitis vectors in Bangladesh, Bhutan, India and Nepal (BBIN), Environmental Health Project, Washigton, DC, USA.
World Health Organization (2006) Guidelines for the Treatment of Malaria. WHO-WC. 770: 12–13.
Amer A, Mehlhorn H. (2006) “Larvicidal effects of various essential oils against Aedes, Anopheles, and Culex larvae (Diptera: Culicidae)”. Parasitol Res. 99: 466–472.
Govindarajan M. (2010) Chemical composition and larvicidal activity of leaf essential oil from Clausena anisata (Willd.) Hook. f. ex Benth (Rutaceae) against three mosquito species. Asian Pac J Trop Med. 3(11): 874–877.
Zhu BCR, Henderson G, Chen F, Fei H, Laine RA. (2001) Evaluation of vetiver oil and seven insect-active essential oils against the Formosan subterranean termite. J Chem Ecol. 27: 1617–1625.
Cheng SS, Chang HT, Chang ST, Tsai KH, Chen WJ. (2003) “Bioactivity of selected plant essential oils against the yellow fever mosquito Aedes aegypti larvae”. Bioresour Technol. 89: 99–102.
Dua VK, Pandey AC, Raghavendra K, Gupta A, Sharma T, Dash AP. (2009) “Larvicidal activity of neem oil (Azadirachta indica) formulation against mosquitoes”. Malar J. 8: 124.
Kannathasan K, Senthilkumar A, Venkatesalu V. (2011) Mosquito larvicidal activity of methyl-p-hydroxybenzoate isolated from the leaves of Vitex trifolia Linn. Acta Trop. 120: 115–118.
O'Neal ST, Johnson EJ, Rault LC, Anderson TD (2019). Vapor delivery of plant essential oils alters pyrethroid efficacy and detoxification enzyme activity in mosquitoes. Pestic Biochem Physiol.;157:88-98.
Mejia-Garibay B, Guerrero-Beltrán JÁ, Palou E, López-Malo A (2015). Características físicas y antioxidantes de semillas y productos de mostaza negra (Brassica nigra) y amarilla (Brassica alba) Physical and antioxidant characteristics of black (Brassica nigra) and yellow mustard (Brassica alba) seeds and their products. Archivos Latinoamericanos de Nutrición 65:128-135.
Anna Grygier (2023): Mustard Seeds as a Bioactive Component of Food, Food 39 (7) 4088–4101.
Flor-Weiler, L.B., Behle, R.W., Berhow, (2023). Bioactivity of brassica seed meals and its compounds as ecofriendly larvicides against mosquitoes. Sci Rep 13, 3936. Reviews International, DOI: 10.1080/87559129.2021.2015774
Feng, R., Isman, M.B (1995). Selection for resistance to azadirachtin in the green peach aphid, Myzus persicae. Experientia 51, 831-833.
Ganesan P, Samuel R, Mutheeswaran S, Pandikumar P, Daniel Reegan AD, Aremu AO, and Ignacimuthu S, (2023) Phytocompounds for mosquito larvicidal activity and their modes of action: A review, South African Journal of Botany,152: 19-49.
O'Neal ST, Johnson EJ, Rault LC, Anderson TD.(2019) Vapor delivery of plant essential oils alters pyrethroid efficacy and detoxification enzyme activity in mosquitoes. Pestic Biochem Physiol. 157,88-98.
Abbott WS. (1925) A method of computing the effectiveness of an insecticide. J Econ Entomol. 1925; 18: 265-267.
Finney DJ,(1971) A statistical treatment of the sigmoid response curve. In: Probit analysis. Cambridge University Press, London; 633.
Manh HD, Huong DNX, Hanh LTH, and Duong NTT. (2019) Rearing of Aedes aegypti mosquitoes in the laboratory and assessing the larvicides of lemongrass oil (Cymbopogon citratus) and lemon eucalyptus oil (Corymbia citriodora) against Aedes aegypti larvae. J. Sci. Lac Hong Univ. 2019; 7: 057-061.
Manh HD, Hue DT, Hieu, NTT, Tuyen DTT, Tuyet OT, and Manh HD. (2020) The Mosquito larvicidal activity of essential oils from Cymbopogon and Eucalyptus Species in Vietnam. Insects 11: 128.
Gebashe FC, Adeyemi O. Aremu, Stephen O. Amoo (2022).Therapeutic Properties of Bioactive Secondary Metabolites in Essential Oil Crops, Therapeutic Use of Plant Secondary Metabolites (2022) 1: 50.
Senthilkumar A, Venkatesalu V(2010). Chemical composition and larvicidal activity of the essential oil of Plectranthus amboinicus (Lour.) Spreng against Anopheles stephensi: a malarial vector mosquito. Parasitol Res. 107(5):1275-8
Govindarajan M (2010). Larvicidal efficacy of Ficus benghalensis L. plant leaf extracts against Culex quinquefasciatus Say, Aedes aegypti L. and Anopheles stephensi L. (Diptera: Culicidae). Eur Rev Med Pharmacol Sci. 14(2):107-11.
Miresmailli, S. and Isman, M.B. (2014) Botanical Insecticides Inspired by Plant-Herbivore Chemical Interactions. Trends in Plant Science, 19, 29-35.
Tao, C., J. Wu, Y. Liu, M. Liu, R. Yang and Z. Lv.(2018). Antimicrobial activities of bamboo (Phyllostachys heterocycla cv. Pubescens) leaf essential oil and its major components. Eur. Food Res. Technol. 244(5): 881-891.
Rajkumar S, Jebanesan A, Nagarajan A. (2011) Effect of leaf essential oil of Coccinia indica on egg hatchability and different larval instars of malarial mosquito Anopheles stephensi. Asian Pac J Trop Med. 4(12): 948–951.
Intirach J, Choochote W, Junkum A, Chaithong U, Champakaew D, Tuetun B, Jitpakdi A, Pitasawat B. (2012) Chemical constituents and combined larvicidal effects of selected essential oils against Anopheles cracens (Diptera: Culicidae) Psyche. 1–11.
Liu P, Xin-Chao L, Hui-Wen D, Zhi-Long L, Shu-Shan D, Zhi-Wei D. (2012) Chemical composition and insecticidal activity of the essential oil of Illicium pachyphyllum fruits against two grain storage Insects. Molecules. 17(12): 14870–14881.
Senthilkumar A, Jayaraman M, Venkatesalu V. (2013) Chemical constituents and larvicidal potential of Feronia limonia leaf essential oil against Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus. Parasitol Res. 112: 1337–1342.
Kristan M, Fleischman H, Della TA, Stich A, Curtis CF. (2003) Pyrethroid resistance/ susceptibility and differential urban/ rural distribution of Anopheles arabiensis and A. gambiae s.s malaria vectors in Nigeria and Ghana. Med Vet Entomol. 17: 326–332.
Siddiqui BS, Rasheed M, Ilyas F, Gulzar T, Tariq RM, Naqvi SNH. (2004) Analysis of Insecticidal Azadirachta indica. Z Naturforsch C. 59: 104–112.
Akpuaka A, Ekwenchi MM, Dashak DA, Dildar A. (2013) Biological Activities of Characterized Isolates of n-Hexane Extract of Azadirachta indica A. Juss (Neem) Leaves. New York Sci J. 6: 119–124
Manas M, Sunita Y, Pawan K, Raka K. (2014) In vitro propagation and biosynthesis of steroidal sapogenins from various morphogenetic stages of Moringa oleifera Lam, and their antioxidant potential. Acta Physiol Plant. 36: 1749–1762.
Ogunlesi M, Okiei W, Ofor E and Osibote A.E (2009). Analysis of the essential oil from the dried leaves of Euphorbia hirta Linn (Euphorbiaceae), a potential medication for asthma. African.J.Biotech. 8: 7042-7050.
Hecht SS (2004). Chemoprevention by Isothiocyanates. In: Kelloff GJ, Hawk ET, Sigman CC, eds. Promising Cancer Chemopreventive Agents, Volume 1: Cancer Chemopreventive Agents. Totowa, NJ: Humana Press: 21-35.
Zhang Y (2004). Cancer-preventive isothiocyanates: measurement of human exposure and mechanism of action. Mutat Res, 555, 173-90.
Sharma A, Sharma A, Yadav P, Singh, D (2016) Isothiocyanates in Brassica: Potential Anti Cancer Agents. Asian Pac J Cancer Prev, 17 (9), 4507-4510.
Khatami M, Soltani N, Pourseyed S (2016). Biogenic synthesis of silver nanoparticles using mustard and its characterization. Int J Nanosci Nanotechnol ;11(4):281–8.
Lafarga T, Bobo G, Viñas I, Collazo C, Aguiló-Aguayo I (2018). Effects of thermal and non-thermal processing of cruciferous vegetables on glucosinolates and its derived forms. J. Food Sci. Technol. ;55(6):1973–1981.
Flor-Weiler LB, Behle RW, Berhow MA, McCormick SP, Vaughn SF, Muturi EJ, Hay WT. Bioactivity of brassica seed meals and its compounds as ecofriendly larvicides against mosquitoes. Sci Rep. 2023 Mar 9;13(1):3936.
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