Bacteriological Profile and Antibiotic Susceptibility in UTI Patient Visiting Antenatal Clinic at A Tertiary Care Hospital
DOI:
https://doi.org/10.63682/jns.v14i4.8877Keywords:
Bacteriological Profile, Antibiotic Susceptibility, UTI, CLSI, IPD, OPDAbstract
Introduction: Urinary tract infections (UTIs) are among the most common infections in humans. Approximately, 150 million UTIs occur every year worldwide, resulting in more than 6 billion US dollars in direct healthcare costs. The emergence of antibiotic-resistant bacterial strains is a serious problem and greatest challenge in public health care.
Aim and Objective: To determine the bacteriological profile along with the antibiotic susceptibility of the isolates in UTI patients visiting antenatal clinic at tertiary care hospital.
Material and Methods: This wasa Observational Cross sectional study carried out in the Departmentof Microbiology at Central Laboratory, Sharda Hospital.The study was conducted on all the urine samples received in bacteriology laboratory of Sharda Hospital. A total of 264 urine sample (including IPD and OPD) were included.The samples were processed according to the biochemicals and theantimicrobial sensitivity testing was performed according to the CLSI guidelines 2024.
Results: In the present study 264 urine samples with significant bacterial colony count i.e. 105 CFU/ml, 172(65.15%) showed growth of Gram-negative bacilli whereas 92(34.84%) were Gram-positive cocci.The age-wise distribution of the bacterial isolates among different pregnancy age groups shows that the maximum number of isolates were received from age group 21-30 with GNB (63.3%) and GPC (36.7%).
Conclusion: There is a critical need for enhanced public health initiatives focusing on sanitation,hydration, and hygiene, alongside holistic clinical management strategies that address both the infectionand its broader health impacts. Future research should aim at developing innovative prevention andtreatment strategies, with a particular focus on high-risk groups such as pregnant women, to mitigate theburden of UTIs in India.
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Bahadi A, El Kabbaj D, Elfazazi H, et al. Urinary tract infection in pregnancy;2022
Lee,A.C.; Mullany,L.C.; Koffi,A.K.;Rafiqullah,I.; Khanam,R.; Folger,L.V.;Rahman, M.; Mitra,D.K.;Labrique,A.;Christian,P.;etal.UrinarytractinfectionsinpregnancyinaruralpopulationofBangladesh:Populationbasedprevalence,riskfactors,etiology,andantibioticresistance.BMCPregnancyChildbirth2020
KariukiS,RevathiG,CorkillJ,KiiruJ,MwituriaJ,MirzaN,HartCA.Escherichia coli from community-acquiredurinary tract infections resistant tofuoroquinolonesandextended-spectrumbeta-lactams.JInfectDevCtries.2007
Study on the Bacteriological Causes of UTI in Pregnant Women and Their Current Resistance Pattern Shivani Gour , Vasundhara Sharma , Imran Ahamad , Umar Farooq , Sudhir Singh , Shweta R Sharma;2022
A Clinico-microbiological Study of Urinary Tract Infections in Pregnant Women attending Antenatal Clinic of a Tertiary-level Hospital with Special Reference to Antimicrobial Sensitivity Pattern Soham Basu,Abirlal Sanyal, Kumkum Bhattacharyya;2024.
Bacteriological profile and anti-biogram pattern of asymptomatic UTI in pregnant Women at Tertiary Care Teaching Hospital, Vadodara, Gujarat Hetal G. Vaghela, Hitesh R. Ahir; 2016
Nicolle LE. Urinary tract infections in special populations: diabetes, renal transplant, HIV infection, and spinal cord injury. Infect Dis Clin North Am. 2014;28:91–104.
Calbo E, Romaní V, Xercavins M, Gómez L, Vidal CG, et al. Risk factors for community-onset urinary tract infections due to Escherichia coli harbouring extended-spectrum beta-lactamases. J Antimicrob Chemother. 2006;57:780–783.
Borah VV, Saikia KK, Chandra P, Hazarika NK, Chakravarty R. New Delhi metallo-β-lactamase and extended spectrum β-lactamases co-producing isolates are high in community-acquired urinary infections in Assam as detected by a novel multiplex polymerase chain reaction assay. Indian J Med Microbiol. 2016;34:173–182.
Saha S, Nayak S, Bhattacharyya I, Saha S, Mandal AK, et al. Understanding the patterns of antibioticsusceptibility of bacteria causing urinary tract infection in West Bengal, India. Front Microbiol. 2014;5:1–7.
Shaikh S, Arora S, Pandya H. A 4-year study of community acquired urinary tract infections from atertiary diagnostic centre in mumbai with special reference to MIC values. J Kidney. 2018;04:04.
Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. 28th ed. M100. Wayne: Clinical and Laboratory Standards Institute (CLSI); 2023. p. 38.
Ranjan A, Sridhar ST, MattaN, et. al; Prevalence of UTI among pregnant women and it’s complications in new borns, Indian Journal of pharmaceutical practice, 2017 Jan.
Ozma MA, Abbasi A, Asgharzadeh M, Pagliano P, Guarino A, Köse Ş, Samadi Kafil H. Antibiotic therapy for pan-drug-resistant infections. Infez Med. 2022 Dec 1;30(4):525-531.
Mahor S, Malviya R, Goyal R. Study of incidence of urinary tract infection during pregnancy and its effect on maternal and perinatal outcome. Int J Reprod Contracept Obstet Gynecol. 2021;10(4):1497-502.
L Thompson et al.; Urinary Tract infections in pregnant individuals, The American college of Obstetricians and Gynaecologists, 2023
KantS, LohiyaA, KapilA, GuptaSK. Urinary tract infection among pregnant women at a secondary level hospital in Northern India. Indian health.2017Apr1
Julius Ojulong et al. Prevalence of Antimicrobial Resistance in Klebsiella pneumoniae, Enterobacter cloacae, and Escherichia coli Isolates among Stillbirths and Deceased Under-Five Children in Sierra Leone: Data from the Child Health and Mortality Prevention Surveillance Sites from 2019 to 2022. Microorganisms. 2024 Aug 13;12(8):1657.
Fatima, Tahira & Rafiq, Sabika & Iqbal, Atia & Hasnain, Shahida. (2020). Prevalence and Antibiogram of MDR E. coli Strains Isolated from UTI Patients—1-Year Retrospective Study at Nishtar Medical Hospital, Multan. SN Comprehensive Clinical Medicine. 2. 10.1007/s42399-020-00246-8.
Wilson ML, Gaido L. Laboratory diagnosis of urinary tract infections in adult patients. Clin Infect Dis. 2004 Apr 15; 38 (8): 1150-8. Epub 2004 Apr 6.
Jennifer L. Young and David E. Soper. Urinalysis and urinary tract infection: update for clinicians. Infect Dis Obstet Gynecol 2001; 9(4):249–255.
Mlugu, E.M., Mohamedi, J.A., Sangeda, R.Z. et al. Prevalence of urinary tract infection and antimicrobial resistance patterns of uropathogens with biofilm-forming capacity among outpatients in Morogoro, Tanzania: a cross-sectional study. BMC. Infect Dis 23, 660 (2023).
G Bonkat T Cai C Galeone B Koves F Bruyere Adherence to European Association of Urology Guidelines and State of the Art of Glycosaminoglycan Therapy for the Management of Urinary Tract Infections: A Narrative Review and Expert Meeting. Report Eur Urol Open Sci2022443745
Javiya VA, Ghatak SB, Patel KR, Patel JA. Antibiotic susceptibility patterns of Pseudomonas aeruginosa at a tertiary care hospital in Gujarat, India. Indian J Pharmacol. 2008 Oct;40(5):230-4.
Bonkat G., Bartoletti R., Bruyère F., et al. European Association of Urology; Arnhem, The Netherlands: 2024. EAU guidelines on urological infections.
Gernot Bonkat et al. Classification of Urinary Tract Infections in 2025: Moving Beyond Uncomplicated and Complicated. Eur Urol Open Sci. 2025 Apr 6; 75:44–47.
Bonkat G, Bartoletti R, Bruyère F, et al. EAU guidelines on urological infections. Arnhem, The Netherlands: European Association of Urology; 2024.
Lee AC, Mullany LC, Koffi AK, et al. Urinary tract infections in pregnancy in rural Bangladesh: prevalence, risk factors, etiology, and antibiotic resistance. BMC Pregnancy Childbirth. 2020;20(1):740.
L Thompson et al. Urinary tract infections in pregnant individuals. Am Coll Obstet Gynecol. 2023.
Julius Ojulong et al. Prevalence of antimicrobial resistance in K. pneumoniae, E. cloacae, and E. coli among stillbirths and deceased children. Microorganisms. 2024;12(8):1657.
Gour S, Sharma V, Ahamad I, et al. Bacteriological causes of UTI in pregnant women and current resistance patterns. 2022.
Basu S, Sanyal A, Bhattacharyya K. Clinico-microbiological profile of UTIs in pregnant women and antimicrobial sensitivity pattern. 2024.
Bonkat G, et al. Classification of urinary tract infections in 2025: moving beyond uncomplicated and complicated. Eur Urol Open Sci. 2025;75:44–47.
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